FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594015
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAACCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC20850.3510012373424914No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19710.331809802782758No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCAC16820.2831578327146621No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA15620.26295632265178487No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14970.2520138380343931No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACAAGATGATCA14880.2504987247796773No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14500.24410157992643283No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT13570.22844540962770302No Hit
GGCTCTGTGAGTTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGGTAAC13490.22709864229017787No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13290.22373172394636498No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12380.20841224548201645No Hit
GTGTATTACTGTGCGAGAGATCGGGTCGATACCAATTACTACCATAGTAG12250.20622374855853806No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12190.20521367305539423No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11930.20083667920843748No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11550.19443953435519304No Hit
CCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG11520.19393449660362114No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGAGTTCCTATGTGCTGACT10880.18316035790341995No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTTCCAATGTTGTTACCCCCACAGGTTA10760.18114020689713223No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10510.17693155896736615No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10330.17390133245793457No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG10040.16901930085940592No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9660.16262215600616148No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9550.16077035091706438No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9470.15942358357953923No Hit
CCTCTCTCACTGCACAGGCTCTGTGAGTTCCTATGTGCTGACTCAGCCAC9320.15689839482167958No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9250.15571997340134508No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTC9170.15437320606381993No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8940.15050124996843514No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8910.1499962122168632No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8800.14814440712776614No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8720.14679763979024096No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8630.1452825265355252No Hit
CGGCCACCCTGACCATCAGCGGGGTCGAAGCCGGGGATGAGGCCGACTAT8370.14090553268856848No Hit
GTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG8310.1398954571854246No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACAAGATGATCAC8300.13972711126823398No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8280.1393904194338527No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG8220.1383803439307088No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT8110.13652853884161173No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC8100.1363601929244211No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGG7650.12878462665084214No Hit
TGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGGCCACCCTGAC7540.12693282156174507No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGAGTTCCTA7470.12575440014141057No Hit
CTATTACTGTCAGGTGTGGGATGGTGATAGTGATCATCTTGTGGTATTTG7460.12558605422421992No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT7420.12491267055545735No Hit
GGCCAGGCCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGG7390.12440763280388543No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7380.12423928688669478No Hit
GGGTGGCCGTGTTCGCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATGGA7320.12322921138355092No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7290.12272417363197899No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7240.12188244404602577No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7230.12171409812883514No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7110.11969394712254741No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7090.11935725528816613No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7010.11801048795064097No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG6880.11582199102716262No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6820.11481191552401876No Hit
GCACAGGCTCTGTGAGTTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA6710.11296011043492168No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC6630.11161334309739654No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAG6620.1114449971802059No Hit
CCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAG6600.1111083053458246No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG6590.11093995942863397No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6510.10959319209110881No Hit
CTTTTACTTCCAATGTTGTTACCCCCACAGGTTACCCTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG6490.10925650025672752No Hit
CTCACTGCACAGGCTCTGTGAGTTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG6360.10706800333324916No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6340.10673131149886789No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6320.10639461966448659No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6230.1048795064097708No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCAGGGTAACCTGTGGGGGTAACAA6230.1048795064097708No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCCGCTGATGGTCAGGGT6120.10302770132067371No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACAAGATGAT6080.10235431765191114No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCCGCTGATGGTCAGGGTGG6070.10218597173472051No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6060.10201762581752986No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6060.10201762581752986No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6010.10117589623157665No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGGTAACCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTG5980.10067085848000472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAA5100.055.11689470
AGGGGGG2250.053.5421270
TCGATTG200.007981854550.6943173
TCTCGTA2250.044.27133638
GGTATCA5350.044.2305151
ATCTCGT2250.042.66807637
TCGTATG2500.041.3774340
TATACAC2650.040.810523
TTATACA2750.039.3265042
CGTATGC2550.039.2813841
CGAGGCT2600.039.1207629
ACGAGAC2650.038.2630822
GTATGCC2750.037.7422642
CACGAGA2700.037.55134621
CTTATAC2800.037.4266851
ATGCCGT2600.037.41636744
CCCACGA2750.036.86859519
GATCTCG2600.036.82680536
TGCTTGA3000.036.8129855
AGACCGA2850.035.60193325
Produced by FastQC (version 0.11.5)