FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594015
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27940.4703584926306575No Hit
GGGTAACCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC20430.3439307088204843No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18990.3196888967450317No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14910.2510037625312492No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13740.23130729021994395No Hit
GGCTCTGTGAGTTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGGTAAC13450.22642525862141527No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCAC13360.22491014536669948No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACAAGATGATCA12910.21733457909312057No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12380.20841224548201645No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA12360.20807555364763516No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12150.20454028938663166No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11890.20016329553967493No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11830.19915322003653105No Hit
GTGTATTACTGTGCGAGAGATCGGGTCGATACCAATTACTACCATAGTAG11290.19006254050823632No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11120.18720065991599538No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10800.1818135905658948No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTTCCAATGTTGTTACCCCCACAGGTTA10630.17895170997365387No Hit
CCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG10350.17423802429231586No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10260.17272291103760007No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGAGTTCCTATGTGCTGACT10150.170871105948503No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9980.16800922535626206No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9730.16380057742649595No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG9430.15875019991077666No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9150.15403651422943865No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8830.14864944487933807No Hit
GGGTGGCCGTGTTCGCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATGGA8640.14545087245271585No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8600.14477748878395327No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTC8570.14427245103238134No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8560.1441041051151907No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8470.14258899186047488No Hit
CCTCTCTCACTGCACAGGCTCTGTGAGTTCCTATGTGCTGACTCAGCCAC8470.14258899186047488No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC8410.14157891635733105No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT8380.14107387860575912No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8170.13753861434475562No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8040.13535011742127726No Hit
GTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG7820.13164650724308308No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG7800.13130981540870182No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGAGTTCCTA7780.1309731235743205No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7700.12962635623679536No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG7640.1286162807336515No Hit
CGGCCACCCTGACCATCAGCGGGGTCGAAGCCGGGGATGAGGCCGACTAT7530.12676447564455443No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGG7490.12609109197579185No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7470.12575440014141057No Hit
CTATTACTGTCAGGTGTGGGATGGTGATAGTGATCATCTTGTGGTATTTG7370.12407094096950413No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7270.12238748179759772No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7080.11918890937097548No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT7020.11817883386783161No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6910.11632702877873455No Hit
TGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGGCCACCCTGAC6890.11599033694435325No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAG6870.11565364510997198No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATACCACAAGATGATCAC6780.11413853185525619No Hit
CCTGTGGGGGTAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAG6690.11262341860054038No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGAGTTCCTATGTGCTGACTCAGC6680.11245507268334974No Hit
GCACAGGCTCTGTGAGTTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA6680.11245507268334974No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC6600.1111083053458246No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6570.11060326759425267No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGAGTTC6530.1099298839254901No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6390.10757304108482109No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6280.10572123599572401No Hit
GGCCAGGCCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGG6270.10555289007853337No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6240.10504785232696144No Hit
CTTTTACTTCCAATGTTGTTACCCCCACAGGTTACCCTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG6220.10471116049258015No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6160.10370108498943628No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6040.10168093398314856No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT5960.10033416664562342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAG200.006093384754.2756760
ATAGCGT200.006748852352.89181550
CATGCGG200.00800836650.65180610
TATTCCG200.00800836650.6518065
GGTATCA5550.041.999361
TCTGGAA4550.040.62687770
CGGGTAC603.4310688E-739.40912217
GTATCAA14500.038.208711
ACGGGTA1151.8189894E-1235.2479116
AGTTAGT400.002650538234.3521238
TATCAAC16400.033.5534932
ATCAACG17000.032.5678373
TCAACGC17100.032.3773844
AACGCAG17500.031.6453176
CAACGCA17600.031.4655155
GACAGGT1400.031.3690871
TAGTTAG450.004764617430.47518237
AGAGTAC18450.030.19890811
ACGCAGA18450.030.0158847
CAGAGTA18700.029.7951810
Produced by FastQC (version 0.11.5)