FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643985
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGG35360.549081112137705No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26900.4177115926613197No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25200.3913134622700839No Hit
CAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA18890.2933298135826145No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18590.2886713199841611No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT15910.2470554438379776No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15540.24130996839988508No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14960.2323035474428752No Hit
GTCTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGGGTGTTAGCAG13820.21460127176875238No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13450.20885579633065984No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13420.20838994697081453No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13240.2055948508117425No Hit
GTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTT13190.20481843521200027No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13150.20419730273220651No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13120.20373145337236115No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12180.18913484009720724No Hit
ACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACAGACTTCACTCTCA10880.16894803450390924No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10810.16786105266427012No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10730.16661878770468255No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10690.16599765522488877No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9980.15497255370854912No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAATGTA9970.15481727058860068No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCC8910.13835725987406539No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8880.13789141051422005No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8850.1374255611543747No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8500.1319906519561791No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT8490.13183536883623065No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTG8390.1302825376367462No Hit
GGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGCCAGG8230.12779800771757108No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8230.12779800771757108No Hit
GTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGC8130.12624517651808662No Hit
CTCCTGCTGGGCCAGTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACC8120.12608989339813814No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8020.1245370621986537No Hit
GTCTGGCACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT8020.1245370621986537No Hit
ATGTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGAC7850.12189724915953011No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7770.12065498419994254No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7720.11987856860020032No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7720.11987856860020032No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7600.11801517116081896No Hit
CTTCCATACTGCTGACAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTG7410.1150647918817985No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7390.11475422564190159No Hit
ATGTATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGCTAAGGAGTTGCTGCTAACAC7380.11459894252195316No Hit
GAGTGAAGTCTGTGCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA7370.11444365940200471No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7340.11397781004215937No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTT7190.11164856324293268No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7080.10994044892349977No Hit
GTATTACTGTGTGAGACGTGGCAGCAACTTTTTTCATTGGGGGCCCTACTACTTTGACTCGTGGGGCCAGGGAAC7040.10931931644370597No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6930.10761120212427308No Hit
CTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC6910.10730063588437619No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC6860.10652422028463396No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6800.10559252156494328No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6740.1046608228452526No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6740.1046608228452526No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6690.10388440724551037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTT200.00787480650.86826310
TCTGTCG200.00787480650.86826310
GACGCGT2400.043.8747726
GGTATCA6650.043.3632281
ACGCGTA2500.042.1328927
TCGTATG2550.041.93978540
GCGTAGT2550.041.3969429
CGTATGC2600.041.1885641
CGCGTAG2500.040.82140428
GTATCAA19200.040.6397361
CGTAGTA2600.040.62618630
TACACAT2850.039.2636835
AGACGCG2800.038.78990625
GCCGTCT2700.038.71125846
CGGTACC459.5734904E-538.62497745
ATGCCGT2700.038.5543944
CGAGACG2750.038.2460923
ATCTCGT2700.038.15423637
CTTATAC2850.038.091621
CACGAGA2850.038.085721
Produced by FastQC (version 0.11.5)