FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643985
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGG35550.5520314914167255No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29790.4625884143264206No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27050.4200408394605465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21490.33370342476921044No Hit
CAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA17760.2757828210284401No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16890.26227318959292534No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15860.24627902823823536No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13540.21025334441019589No Hit
GTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTT13200.20497371833194875No Hit
GTCTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGGGTGTTAGCAG13120.20373145337236115No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12980.2015574896930829No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12740.1978306948143202No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12060.1872714426578259No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11670.1812154009798365No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAATGTA11140.17298539562256884No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10880.16894803450390924No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10800.1677057695443217No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10720.1664635045847341No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10600.16460010714535275No Hit
ACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACAGACTTCACTCTCA10490.16289199282591987No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10210.15854406546736338No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9930.1541961381088069No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9640.14969292763030195No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9420.14627669899143614No Hit
GGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGCCAGG9240.1434816028323641No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8860.13758084427432316No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCC8560.13292235067586977No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT8310.12904027267715862No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8300.12888498955721017No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8170.1268663089978804No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8020.1245370621986537No Hit
GTATTACTGTGTGAGACGTGGCAGCAACTTTTTTCATTGGGGGCCCTACTACTTTGACTCGTGGGGCCAGGGAAC7920.12298423099916923No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7750.12034441796004566No Hit
ATGTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGAC7710.11972328548025186No Hit
CTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC7680.11925743612040654No Hit
GTCTGGCACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT7590.11785988804087053No Hit
CTCCTGCTGGGCCAGTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACC7510.11661762308128294No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7510.11661762308128294No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTG7460.11584120748154073No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7190.11164856324293268No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7130.110716864523242No Hit
GAGTGAAGTCTGTGCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA7100.11025101516339665No Hit
ATGTATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGCTAAGGAGTTGCTGCTAACAC7090.11009573204344822No Hit
GTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGC7050.10947459956365443No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTT7010.10885346708386064No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6890.10699006964447931No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6860.10652422028463396No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6770.10512667220509794No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC6730.10450553972530417No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6690.10388440724551037No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA6670.1035738410056135No Hit
CTTCCATACTGCTGACAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTG6600.10248685916597437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.042.785151
GTATCAA19900.039.295521
GGCTGAT700.001559207238.26862770
TCGGGTC400.002410055835.02520848
TATCAAC22750.033.3027152
AACGCAG23550.032.4687426
ATCAACG23600.032.1032523
TCAACGC23850.032.0478824
CAACGCA24050.031.645665
ACGCAGA24500.031.2097537
TCATTCG553.6498302E-430.7578018
CGCAGAG25000.030.720898
AGAGTAC25000.030.45022211
ATAATAC450.00509147230.064953
TAATACT902.3724533E-730.0626184
GCAGAGT25700.030.0157819
GAGTACT7050.028.79453512
AGTACTT7200.028.1990313
GCGTAGA12000.027.90420570
CAGAGTA27950.027.35738610
Produced by FastQC (version 0.11.5)