FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715977
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTATACTATTGTGTCAAAGATCAGCGTTTTGCAGTGGGAGCCCAGGG27550.3847888968500385No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22720.3173286292716107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20990.293165841919503No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20530.2867410545310813No Hit
GTATAGGGCCGTGTCGTCGGCTCTCAGGCTGCTCATTTGCAGATATAGAG20130.2811542828889755No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17320.24190721210318208No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT16690.23310804676686545No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16560.23129234598318105No Hit
GTATTAGTGCTGGTGGTAGTACCACACACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT15640.21844277120633762No Hit
ATACTATTGTGTCAAAGATCAGCGTTTTGCAGTGGGAGCCCAGGGAACCCTAGTCACCGTCTCCTCAGCTTCCAC15520.21676673971370589No Hit
CAATAGTATAGGGCCGTGTCGTCGGCTCTCAGGCTGCTCATTTGCAGATA14610.20405683422791515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14360.20056510195159902No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14250.1990287397500199No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14170.19791138542159872No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13430.18757585788370296No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13130.18338577915212362No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12900.18017338545791275No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA12540.17514529098001752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12470.174167605942649No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACAG12470.174167605942649No Hit
CTATAATGGGACGTTCGGCCAAGGGACCAAGGTGGACGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT12460.17402793665159635No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAG12320.1720725665768593No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12300.17179322799475402No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12150.16969818862896435No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCCAGAACACTCTATATCTGCAAATGAG12070.1685808343005432No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12060.16844116500949052No Hit
ATAATGGGACGTTCGGCCAAGGGACCAAGGTGGACGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC11860.16564777918843762No Hit
TCTATGAGGCGTCTAATTTAGAAAGTGGGGTTCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11000.1536362201579101No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10940.15279820441159422No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10920.15251886582948893No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10830.15126184221001512No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT10780.1505634957547519No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10770.15042382646369926No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGATTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAA10660.14888746426212016No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10630.1484684563889622No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG10370.14483705482159343No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACTTACTA10360.1446973855305408No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG10170.14204366900054052No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10140.14162466112738256No Hit
GATATAGAGTGTTCTGGGAATTGTCTCTGGAGATGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTGTGTGGTA10010.1398089603436982No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATATTGCAACTTA9990.1395296217615929No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC9750.13617755877632942No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT9750.13617755877632942No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9590.13394285011948706No Hit
GACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9340.13045111784317096No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA9300.12989244067896036No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9260.1293337635147498No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGA9210.12863541705948656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9160.12793707060422332No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG9110.1272387241489601No Hit
CTCTATATCTGCAAATGAGCAGCCTGAGAGCCGACGACACGGCCCTATACTATTGTGTCAAAGATCAGCGTTTTG9090.1269593855668548No Hit
GATTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTCATCTATGAGGCGT8890.12416599974580189No Hit
GGGGAGGTGTGTCACAAGATTTGGGCTCTGGGCACCGTGGGCATGTGTGA8760.12235029896211749No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8760.12235029896211749No Hit
CCTTTAGCACCTACGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCCGCAGTGGGTCTCCAGTATTAGTG8600.12011559030527517No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8590.11997592101422254No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACGT8560.11955691314106459No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8310.11606518086474844No Hit
CTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG8300.11592551157369581No Hit
GGACCAAGGTGGACGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8200.11452881866316936No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA8190.1143891493721167No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8170.11410981079001141No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8130.11355113362580083No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC8110.11327179504369554No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC8040.11229411000632701No Hit
GCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGCCTCTGGATTCACCTTTAGCACCTACGCCATGAGCTGGGTCCG7870.10991973205843204No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC7790.10880237773001089No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGATGTTCCCCTGCTGCCACCTGCTCTTGTCCACGGTGAG7770.10852303914790559No Hit
ATAGTATAGGGCCGTGTCGTCGGCTCTCAGGCTGCTCATTTGCAGATATA7730.10796436198369501No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7730.10796436198369501No Hit
TCCCATTATAGTGTTGGCAGTAATAAGTTGCAATATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT7690.10740568481948443No Hit
CCCCTAACCTCCTCATCTATGAGGCGTCTAATTTAGAAAGTGGGGTTCCG7650.10684700765527386No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7630.10656766907316856No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGCCTCTGG7590.10600899190895798No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTCAAAGGTATCCA7500.10475196828948416No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACGTCCACCTTGGTCC7490.1046122989984315No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7450.10405362183422093No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT7430.10377428325211563No Hit
GTCTGCGTAGTGTGTGGTACTACCACCAGCACTAATACTGGAGACCCACT7420.103634613961063No Hit
CACTATAATGGGACGTTCGGCCAAGGGACCAAGGTGGACGTCAAACGAACTGTGGCTGCACCATCTGTCTTCAT7350.10265692892369446No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7330.10237759034158918No Hit
GTGCAGCCACAGTTCGTTTGACGTCCACCTTGGTCCCTTGGCCGAACGTC7320.10223792105053654No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACGTCCAGATGACCCAGTCTCCTTCCACC7290.1018189131773786No Hit
CCATTATAGTGTTGGCAGTAATAAGTTGCAATATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC7240.10112056672211538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1150.051.1090141
TCGTATG1200.048.9103740
ATGCCGT1300.045.3174444
ATCTCCG1300.044.7034610
GTGGTAT1200.042.749161
GAGCTAC1450.042.53755630
TCAGCTT11400.040.95673870
GGAGCTA1450.040.160329
GTATCAA11500.040.147041
AGACGGA1450.040.11818725
CCCACGA1450.040.08736419
TATACAC1450.040.0789643
CTCGTAT1500.039.08694539
GTACGGA702.3626853E-839.0849461
GTATGCC1600.038.92019742
CTTATAC1500.038.759241
ACACATC1500.038.7436
TCTCCGA1500.038.74311
TAACGGC451.01738486E-438.22895436
GCCGTCT1550.038.1134146
Produced by FastQC (version 0.11.5)