FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715977
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTATACTATTGTGTCAAAGATCAGCGTTTTGCAGTGGGAGCCCAGGG26890.3755707236405639No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21270.2970765820689771No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20850.29121047184476595No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT19070.266349338037395No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16810.23478407825949718No Hit
GTATAGGGCCGTGTCGTCGGCTCTCAGGCTGCTCATTTGCAGATATAGAG16730.23366672393107601No Hit
ATACTATTGTGTCAAAGATCAGCGTTTTGCAGTGGGAGCCCAGGGAACCCTAGTCACCGTCTCCTCAGCTTCCAC15940.22263284993791702No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15790.22053781057212735No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15440.21564938538528472No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15050.21020228303423155No Hit
GTATTAGTGCTGGTGGTAGTACCACACACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT14950.2088055901237051No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14940.20866592083265242No Hit
CTCTATATCTGCAAATGAGCAGCCTGAGAGCCGACGACACGGCCCTATACTATTGTGTCAAAGATCAGCGTTTTG14750.20601220430265219No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14180.1980510547126514No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13450.18785519646580826No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT12570.17556429885317545No Hit
ATAATGGGACGTTCGGCCAAGGGACCAAGGTGGACGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC12180.17011719650212226No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCCAGAACACTCTATATCTGCAAATGAG12170.16997752721106962No Hit
CAATAGTATAGGGCCGTGTCGTCGGCTCTCAGGCTGCTCATTTGCAGATA12010.16774281855422732No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11880.16592711777054292No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11630.16243538549422676No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA11540.16117836187475296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11530.16103869258370032No Hit
CTATAATGGGACGTTCGGCCAAGGGACCAAGGTGGACGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT11510.16075935400159502No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAG11490.16048001541948972No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11400.15922299180001592No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10940.15279820441159422No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10680.14916680284422545No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10650.1487477949710675No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACAG10490.14651308631422516No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10470.1462337477321199No Hit
TCTATGAGGCGTCTAATTTAGAAAGTGGGGTTCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT10080.1407866453810667No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9990.1395296217615929No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9910.13841226743317175No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG9760.13631722806738206No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACTTACTA9610.13422218870159236No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9580.13380318082843443No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATATTGCAACTTA9520.13296516508211856No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9340.13045111784317096No Hit
GATATAGAGTGTTCTGGGAATTGTCTCTGGAGATGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTGTGTGGTA9330.1303114485521183No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9260.1293337635147498No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9250.12919409422369713No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA9130.1275180627310654No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG9090.1269593855668548No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT9070.12668004698474952No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTT9020.12598170052948626No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9010.12584203123843363No Hit
CCTTTAGCACCTACGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCCGCAGTGGGTCTCCAGTATTAGTG8990.12556269265632836No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8990.12556269265632836No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8940.12486434620106511No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGA8700.12151228321580163No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8670.12109327534264369No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGATTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAA8640.12067426746948576No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC8620.12039492888738046No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8610.12025525959632782No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8590.11997592101422254No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT8510.11885856668580135No Hit
CCATTATAGTGTTGGCAGTAATAAGTTGCAATATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC8330.11634451944685373No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGATGTTCCCCTGCTGCCACCTGCTCTTGTCCACGGTGAG8190.1143891493721167No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8110.11327179504369554No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8090.11299245646159024No Hit
GGGGAGGTGTGTCACAAGATTTGGGCTCTGGGCACCGTGGGCATGTGTGA7910.11047840922264263No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA7810.10908171631211618No Hit
TCCCATTATAGTGTTGGCAGTAATAAGTTGCAATATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT7810.10908171631211618No Hit
GTGCAGCCACAGTTCGTTTGACGTCCACCTTGGTCCCTTGGCCGAACGTC7700.10754535411053706No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGAACCCCA7690.10740568481948443No Hit
GATTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTCATCTATGAGGCGT7680.10726601552843178No Hit
ACCACACACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCCAGAACACTCTATATCTG7660.10698667694632649No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACGT7580.10586932261790533No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG7530.10517097616264211No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7530.10517097616264211No Hit
CTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG7430.10377428325211563No Hit
GCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGCCTCTGGATTCACCTTTAGCACCTACGCCATGAGCTGGGTCCG7430.10377428325211563No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA7400.10335527537895771No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7320.10223792105053654No Hit
GTCTGCGTAGTGTGTGGTACTACCACCAGCACTAATACTGGAGACCCACT7320.10223792105053654No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC7290.1018189131773786No Hit
GGACCAAGGTGGACGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7280.10167924388632596No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA7260.10139990530422067No Hit
CCTCATAGATGAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACTTACTAATAATCTGG7240.10112056672211538No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7160.10000321239369422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG200.006434362453.53502361
ACTGTCG200.00766896251.2108321
TAGCTCG200.007696554551.16433310
TTACCAT400.00927400548.80211370
TACTGGT306.6715316E-445.4825822
TCGCATT350.001421099939.00414318
GTATCAA14000.036.0914421
GCCTTAT400.00256525534.58299642
GTAGCTC501.9894063E-434.1095549
TCAGCTT11100.034.0002770
ACAGGTA959.731593E-932.343681
CAGGTAT553.4790635E-431.0108532
ACTTGTC553.4804884E-431.0086868
ACGGATT850.004652607330.62093570
TGAGCGG1303.459325E-530.0320770
ATCAACG16650.029.9057033
GTGGTAT2200.029.4850271
TCAACGC16900.029.463314
AACGCAG16900.029.2655936
TATCAAC17300.029.1824672
Produced by FastQC (version 0.11.5)