FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865159
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30980.3580844677105596No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16280.18817350336758906No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16120.18632413232712136No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15370.177655205574929No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14530.16794600761247355No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13530.1563874386095504No Hit
GTCCTGGGCCCAGCCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGGCAGGAGGTCACCATCTCCTG12930.1494522972077965No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12790.14783409754738724No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12670.14644706926703646No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12330.14251715580604257No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11990.1385872423450487No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11650.13465732888405485No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10860.12552605937174555No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10380.11997794625034242No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10320.11928443211016704No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACTCCAGCACCCAGG10210.11801298951984548No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9960.11512334726911469No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9620.11119343380812081No Hit
GTGTATTACTGTGCGAGACATCGGAGGGACAAGTTTTGGAGTGGTCTTTG9490.1096908198377408No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9420.10888172000753618No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9190.10622324913686387No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGGTTCAGGGAGA9160.10587649206677617No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9090.10506739223657155No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8960.10356477826619154No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8800.10171540722572384No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG8680.10032837894537305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4250.044.7030371
GGTATCA13600.041.9091031
TGGTATC4800.039.548792
GTATCAA37800.034.5546841
TATCAAC40150.032.08372
ATCAACG42350.030.3317073
AACGCAG43050.030.001176
TCAACGC42800.029.933614
CAACGCA43350.029.5555365
CGCAGAG44800.028.8292488
ACGCAGA44900.028.765047
AGAGTAC45450.028.26777811
CAGAGTA46050.027.89947110
GCAGAGT46400.027.8351389
TTATATC550.00916554926.65524365
TACGGAT3450.026.33693170
GTGCTAG1501.2660166E-924.8793721
GTGTTAG1001.7796945E-523.7484911
TGGGGCA6050.023.60062870
GAGTACG41350.022.79059212
Produced by FastQC (version 0.11.5)