FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332223
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTACATTTGCAGATACA49460.37125916607054527No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39010.29281884489308474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37000.2777312807240229No Hit
GTGTATTACTGTGCGAGAGATCGGGACGTGGTGACTAAGAACTACTTTGC29460.22113414946296528No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAACAGAAACCAGGGGAAGCCCCTAAGCTCCTGATCTATGGTGCATC26710.20049195967942304No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT25580.19200989624109477No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25570.19193483373279097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24310.18247695768651345No Hit
GGCTAACAGTTTCCCTCAGACGTTCGGCCAAGGGACCGAGGTGGAGATCAAACGAACTGTGGCTGCACCAT23610.17722258210524816No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22360.1678397685672744No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22320.16753951853405924No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA22170.16641358090950237No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22050.1655128308098569No Hit
GTAGGAGACAGAGTCACTATCACCTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAACAGAAA21480.16123426783654088No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20280.15222676684008607No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC20070.1506504541657065No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19850.14899907898302311No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19720.14802326637507385No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19570.146897328750517No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19140.14366964089345402No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19120.14351951587684644No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19020.14276889079380856No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18660.1400666404948721No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18660.1400666404948721No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18330.13758957772084704No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18320.13751451521254324No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18050.1354878274883409No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTACATTTGCAGATA17850.13398657732226513No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17690.13278557718940448No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17260.12955788933234152No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17250.12948282682403772No Hit
CCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTCTGTTGATACCAGGC17190.12903245177421496No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16710.12542945137563308No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16430.12332770114312694No Hit
CTCTTGTTCAGTCTCTGGATTCACCTTCAGGAGTTATAGTATGAACTGGGTCCGCCAGGTTCCAGGGAAGGGG16420.12325263863482315No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG16410.12317757612651935No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15950.11972470074454501No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15820.11874888813659575No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15790.11852370061168438No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15600.11709751295391237No Hit
GTATTACTGTGCGAGAGATCGGGACGTGGTGACTAAGAACTACTTTGCCTACTGGGGCCAGGGAACCCTGGTCAC15480.11619676285426689No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15410.11567132529614035No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTCGGTCCCTTGGCCGAACGTCTGAGGGAAA14910.11191819988095085No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14860.1115428873394319No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTCGGTCCCTTGGCCGAACGTC14720.11049201222317886No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14720.11049201222317886No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14300.10733938687441967No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT14090.10576307420004008No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14070.1056129491834325No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14070.1056129491834325No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13980.10493738660869839No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC13880.10418676152566049No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13760.10328601142601503No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13700.10283563637619228No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13660.10253538634297711No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13400.10058376112707858No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13350.10020844858555963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG4800.043.8635240
CGTATGC4900.042.3189641
TATACAC4900.041.4930083
ATGCCGT5000.040.98690844
TTATACA5050.040.260542
TCTGGAG16650.040.06862370
CTTATAC5000.040.0034451
CCCACGA5000.039.99293519
GTGGTAT3400.039.883791
ATCTCGT4650.039.127937
CGAGACT5300.039.0315823
CTTGAAA5700.038.85642657
GCCGTCT5300.038.7832846
ACTAGGC5450.038.6405327
GTATGCC5400.038.45500642
CTCTTAT1500.038.4213871
TCTCCGA5300.038.362911
GAGACTA5400.038.3159724
CACGAGA5350.038.01863521
ATCTCCG5350.038.00436410
Produced by FastQC (version 0.11.5)