FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332223
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52020.3904751681963155No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTACATTTGCAGATACA42200.3167637850419937No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37190.27915746838179495No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32000.2402000265721279No Hit
GTGTATTACTGTGCGAGAGATCGGGACGTGGTGACTAAGAACTACTTTGC29560.22188477454600317No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26030.19538770911476533No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAACAGAAACCAGGGGAAGCCCCTAAGCTCCTGATCTATGGTGCATC23480.1762467694972989No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22180.16648864341780617No Hit
GGCTAACAGTTTCCCTCAGACGTTCGGCCAAGGGACCGAGGTGGAGATCAAACGAACTGTGGCTGCACCAT21530.1616095803780598No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20710.15545445469714905No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA19890.14929932901623827No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19720.14802326637507385No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19630.14734770380033974No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19520.14652201620899805No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC19120.14351951587684644No Hit
GTAGGAGACAGAGTCACTATCACCTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAACAGAAA19110.14344445336854267No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18320.13751451521254324No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18260.1370641401627205No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17880.13421176484717648No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17820.13376138979735375No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17330.13008332689046803No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17310.12993320187386045No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17160.1288072642493036No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16880.1267055140167975No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16850.12648032649188612No Hit
CTCTTGTTCAGTCTCTGGATTCACCTTCAGGAGTTATAGTATGAACTGGGTCCGCCAGGTTCCAGGGAAGGGG16740.12565463890054443No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16640.12490401381750653No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16520.12400326371786105No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16420.12325263863482315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16260.12205163850196252No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16020.12025013830267155No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16000.12010001328606396No Hit
GTATTACTGTGCGAGAGATCGGGACGTGGTGACTAAGAACTACTTTGCCTACTGGGGCCAGGGAACCCTGGTCAC15750.11822345057846922No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTCGGTCCCTTGGCCGAACGTC15560.11679726292069721No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTACATTTGCAGATA15400.11559626278783658No Hit
CCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTCTGTTGATACCAGGC15260.11454538767158352No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15200.11409501262176078No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15180.11394488760515319No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14980.1124436374390774No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14890.11176807486434329No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14760.11079226225639402No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG14760.11079226225639402No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14730.11056707473148264No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14650.10996657466505232No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14510.10891569954879926No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14490.10876557453219168No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14240.10688901182459692No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTCGGTCCCTTGGCCGAACGTCTGAGGGAAA13800.10358626145923018No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13730.10306082390110363No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13550.10170969875163542No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13510.10140944871842025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)