FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721068
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25560.35447419660836427No Hit
GAGTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT23880.3311754231223685No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22530.31245319442826475No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17480.24241819079476556No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17310.2400605768110636No Hit
CTTTTGGCCAGGGGACCGAGCTGGAGATCAAACGAACTGTGGCTGCACCA17000.23576139837019533No Hit
TTATAATACTTATTCTCATGTGTACACTTTTGGCCAGGGGACCGAGCTGG16800.23298773485995772No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16550.22952065547216075No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16050.22258649669656674No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15720.21800995190467473No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15440.21412682299034208No Hit
GTATTATAATGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC15040.2085794959698669No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14550.2017840203697848No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14490.20095192131671355No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14000.19415644571663143No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13910.19290829713702454No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12960.17973339546339598No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12590.1746021179694564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12090.16766795919386243No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT11610.16101116676929222No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11010.15269017623857944No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA10990.15241280988755568No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10430.14464655205889043No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10310.14298235395274786No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA10090.1399313240914865No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10050.139376591389439No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9770.13549346247510638No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9770.13549346247510638No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9630.13355189801794004No Hit
GTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT9150.12689510559336983No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9070.1257856401892748No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9060.1256469570137629No Hit
GGCCAAAAGTGTACACATGAGAATAAGTATTATAATGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGG9040.12536959066273917No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9010.12495354113620351No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8910.12356670938108473No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC8880.12315065985454908No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC8610.11940621411572834No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8390.11635518425446698No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAA8250.11441361979730068No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8200.11372020391974128No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTCGGTCC8100.11233337216462248No Hit
TGTGTACACTTTTGGCCAGGGGACCGAGCTGGAGATCAAACGAACTGTGG8100.11233337216462248No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTAGTA8030.11136258993603933No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7900.1095597086543849No Hit
CCTTCCACCCTTTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG7700.10678604514414729No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG7650.1060926292665879No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7650.1060926292665879No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTAGTAATACTCTGA7630.10581526291556413No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7590.10526053021351661No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7540.10456711433595722No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7400.1026255498787909No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7380.10234818352776714No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7350.10193213400123151No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTCGGTCCCCTGGCCAAAAGTG7330.10165476765020774No Hit
CTAGTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGAAAGGGTGGAAGGAGA7260.1006839854216246No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7230.10026793589508895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGT150.002517728867.8483621
GTCGATT200.00605691454.35938662
ATCGTTC200.00645335753.49515556
GACGGTA200.00787280950.872158
TCGTATG2700.049.7645440
CGTATGC2750.047.70101541
GTATGCC3000.046.10866542
CTTATAC3300.044.21651
ATGCCGT3050.043.2595244
ATCTCCG3300.043.16424610
CGCTCAT3400.043.00853328
CGAGACG3250.042.81447223
CGAGCCC3250.042.78181515
ATCTCGT3050.042.75858737
TCTCCGA3350.042.52000411
TATACAC3450.042.2647133
GAGACGC3400.040.93128224
ACGAGAC3400.040.91424622
TTATACA3650.040.8778842
GACGCTC3500.040.7485926
Produced by FastQC (version 0.11.5)