FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721068
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26760.37111617766978977No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24930.34573715655111587No Hit
GAGTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT21360.2962272628933748No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19970.27695030149722355No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19960.2768116183217117No Hit
TTATAATACTTATTCTCATGTGTACACTTTTGGCCAGGGGACCGAGCTGG17260.2393671609335042No Hit
CTTTTGGCCAGGGGACCGAGCTGGAGATCAAACGAACTGTGGCTGCACCA16400.22744040783948255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15670.21731653602711534No Hit
GTATTATAATGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC15120.20968896137396198No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14890.20649924833718872No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14380.19942640638608283No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14080.19526591112072647No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14010.19429512889214332No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13410.18597413836143056No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13340.18500335613284738No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12230.16960952365102877No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12160.1686387414224456No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11820.1639235134550417No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11660.1617045826468516No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11150.15463174069574576No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11130.154354374344722No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10520.14589470063849735No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA10450.1449239184099142No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10060.13951527456495086No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9930.13771239328329643No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9880.13701897740573704No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9850.13660292787920142No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA9670.13410663071998757No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA9630.13355189801794004No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9530.13216506626282126No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC9050.12550827383825103No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8930.1238440757321085No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAA8870.12301197667903721No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8680.1203769963443115No Hit
GTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT8510.11801938236060953No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTAGTA8450.11718728330753826No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8440.11704860013202639No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8270.11469098614832443No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8270.11469098614832443No Hit
GGCCAAAAGTGTACACATGAGAATAAGTATTATAATGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGG8260.11455230297281255No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC8100.11233337216462248No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8080.11205600581359872No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7940.11011444135643239No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTAGTAATACTCTGA7940.11011444135643239No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7810.10831156007477796No Hit
GTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG7780.10789551054824233No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7730.10720209467068294No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7500.1040123816339097No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7410.1027642330543028No Hit
TGTGTACACTTTTGGCCAGGGGACCGAGCTGGAGATCAAACGAACTGTGG7350.10193213400123151No Hit
CTAGTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGAAAGGGTGGAAGGAGA7320.10151608447469587No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTCGGTCC7230.10026793589508895No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7230.10026793589508895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG601.4159998E-534.2300235
GTATCAA20050.033.150341
GCTTGTG1209.458745E-1131.0854931
ATCAACG22750.028.8737753
TCAACGC22950.028.7696864
TATCAAC23050.028.6488482
AACGCAG23200.028.6056166
CAACGCA23100.028.5828725
TGTTAGA8500.028.24015470
CGCAGAG24400.026.9212448
ACGCAGA24850.026.8425057
AGAGTAC24600.026.84187511
GTTTAAC907.4887867E-626.5526133
GCAGAGT25200.026.068419
GTGGTAT3000.025.9987741
ACGGGTC2250.025.59176416
CGGTACC851.3553585E-424.57762145
CGACTGT1001.8006585E-523.71166219
CACATGT1052.5686813E-522.64695428
GTGCTAG1202.851566E-622.607631
Produced by FastQC (version 0.11.5)