FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418166
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCGCTGTCCTCGGTCCTCAGGCTGTTCATTTGCAGAAACA11140.26640138126963936No Hit
GGACTGAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9130.21833434569046742No Hit
CTTTTGGACAGGGGACCAAACTGGAGATCAGACGAACTGTGGCTGCACCA8870.2121167191976392No Hit
GTTTATTACTGCGCGAAAGAGGGGACGCCGAGCTATCCACATAAACCGAG8530.20398597686086387No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8340.1994423267314894No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8020.19178986335570083No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7710.18437653946040566No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7520.1798328893310312No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7480.17887633140905765No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7130.1705064495917889No Hit
CCCTAAGACTCTCCTGTGTAGCCTCTGGATTCACATTTAATAGTTATGTC6900.16500624154044086No Hit
CCATAGATCAGGGACTTCGGGACTTTCCCGGCCTTCTGTTGAAACCAGAC6880.1645279625794541No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC6710.16046259141106642No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT6670.15950603348909284No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT6350.15185357011330428No Hit
GTATGGGGTGCCACCACTACCACTGATGCCTGCGACCCACTCCGGCCCCTTCCCTGGAGCCTGGCGGACCCACGA6340.1516144306328109No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC6180.14778819894491663No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6050.1446793856985025No Hit
GTAATATATTGCAAAATCTTCAGGCTGCAGGCCGGTGATGGTTAGAGTGAAATCTGTCCCGCCTCCACTGCCA5730.13702692232271393No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5730.13702692232271393No Hit
GTCTCCATCCTCACTGACTGCATCTGTAGGAGAAACCGTCACCATCACTTGTCGGGCGAGTCGGGGAATTGGTA5720.13678778284222057No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5710.13654864336172717No Hit
CAGTAATATATTGCAAAATCTTCAGGCTGCAGGCCGGTGATGGTTAGAGTGAAATCTGTCCCGCCTCCACTGCCA5630.13463552751778002No Hit
ATACTACGCAGAGTCCGTGAAGGGCCGGTTCACCGTCTCCAGAGACTCCT5600.13391810907629986No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5530.1322441327128461No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG5510.1317658537518593No Hit
GATCTATGGTGCATCCAGTTCGCAGAGTGGGGTCCCGAGAAGGATCAGTGGCAGTGGAGGCGGGACAGATTTCA5490.13128757479087252No Hit
ATATTACTGTCAACAATATGATGTTTTCCCGTACACTTTTGGACAGGGGA5300.12674392466149806No Hit
GTCCTCAGGCTGTTCATTTGCAGAAACAGTGTGTTCTTGGAGGAGTCTCT5290.1265047851810047No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC5240.1253090877785377No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5190.12411339037607075No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC5190.12411339037607075No Hit
CTCCTGTGTAGCCTCTGGATTCACATTTAATAGTTATGTCATGTCGTGGG5170.12363511141508397No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC5120.122439414012617No Hit
GCCTGAGGACCGAGGACAGCGCCGTTTATTACTGCGCGAAAGAGGGGACG4960.1186131823247227No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAAACGGTGACCTGCGTTCCCTGG4900.11717834544176235No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4860.1162217875197888No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA4840.115743508558802No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4800.11478695063682845No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT4800.11478695063682845No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT4790.11454781115633504No Hit
GAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGT4720.1128738347928813No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT4710.1126346953123879No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT4700.11239555583189452No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC4690.11215641635140111No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4690.11215641635140111No Hit
GTGGTAGTGGTGGCACCCCATACTACGCAGAGTCCGTGAAGGGCCGGTTC4690.11215641635140111No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4680.11191727687090772No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4600.11000416102696059No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA4550.10880846362449363No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGATGCGATGTCCAGATGACCCAGTCTCCATCCTCA4440.10617792933906631No Hit
GATCAGGACTGAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGC4420.10569965037807952No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTCAGGTGCAACTGTTGGAAT4390.10498223193659935No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4380.10474309245610594No Hit
ACCCCATACTACGCAGAGTCCGTGAAGGGCCGGTTCACCGTCTCCAGAGACTCCTCCAAGAACACACTGTTTCTG4350.10402567401462577No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC4340.10378653453413236No Hit
GTCCAAAAGTGTACGGGAAAACATCATATTGTTGACAGTAATATATTGCA4340.10378653453413236No Hit
GTCTTAGGGACCCCCCAGGCTGTACCAAGCCTCCCCCAGATTCCAACAGT4290.10259083713166542No Hit
GCGTAGTATGGGGTGCCACCACTACCACTGATGCCTGCGACCCACTCCGGCCCCTTCCCTGGAGCCTGGCGGACC4260.10187341869018524No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA4190.10019944232673149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)