FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418166
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14620.34962192048133994No Hit
GTAATAAACGGCGCTGTCCTCGGTCCTCAGGCTGTTCATTTGCAGAAACA11260.26927105503556004No Hit
GGACTGAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9570.2288564828321767No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8040.19226814231668765No Hit
GTTTATTACTGCGCGAAAGAGGGGACGCCGAGCTATCCACATAAACCGAG8020.19178986335570083No Hit
CTTTTGGACAGGGGACCAAACTGGAGATCAGACGAACTGTGGCTGCACCA7760.18557223686287264No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7580.18126772621399156No Hit
CCCTAAGACTCTCCTGTGTAGCCTCTGGATTCACATTTAATAGTTATGTC7040.16835419426734838No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7040.16835419426734838No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6920.16548452050142767No Hit
CCATAGATCAGGGACTTCGGGACTTTCCCGGCCTTCTGTTGAAACCAGAC6520.15591894128169195No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT6460.15448410439873161No Hit
GTATGGGGTGCCACCACTACCACTGATGCCTGCGACCCACTCCGGCCCCTTCCCTGGAGCCTGGCGGACCCACGA5850.13989659608863464No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5730.13702692232271393No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA5700.13630950388123375No Hit
GTAATATATTGCAAAATCTTCAGGCTGCAGGCCGGTGATGGTTAGAGTGAAATCTGTCCCGCCTCCACTGCCA5690.13607036440074038No Hit
GTCTCCATCCTCACTGACTGCATCTGTAGGAGAAACCGTCACCATCACTTGTCGGGCGAGTCGGGGAATTGGTA5670.1355920854397536No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC5650.1351138064787668No Hit
CAGTAATATATTGCAAAATCTTCAGGCTGCAGGCCGGTGATGGTTAGAGTGAAATCTGTCCCGCCTCCACTGCCA5590.13367896959580644No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC5550.13272241167383286No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5520.1320049932323527No Hit
ATATTACTGTCAACAATATGATGTTTTCCCGTACACTTTTGGACAGGGGA5380.1286570405054452No Hit
GAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGT5240.1253090877785377No Hit
GATCTATGGTGCATCCAGTTCGCAGAGTGGGGTCCCGAGAAGGATCAGTGGCAGTGGAGGCGGGACAGATTTCA5230.12506994829804433No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG5210.12459166933705752No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5190.12411339037607075No Hit
ATACTACGCAGAGTCCGTGAAGGGCCGGTTCACCGTCTCCAGAGACTCCT5120.122439414012617No Hit
CTCCTGTGTAGCCTCTGGATTCACATTTAATAGTTATGTCATGTCGTGGG5120.122439414012617No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAAACGGTGACCTGCGTTCCCTGG5050.12076543764916324No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT5050.12076543764916324No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5020.12004801920768307No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT4920.11765662440274915No Hit
GTCCTCAGGCTGTTCATTTGCAGAAACAGTGTGTTCTTGGAGGAGTCTCT4820.11526522959781522No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT4820.11526522959781522No Hit
GCCTGAGGACCGAGGACAGCGCCGTTTATTACTGCGCGAAAGAGGGGACG4710.1126346953123879No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC4620.11048243998794736No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT4600.11000416102696059No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4600.11000416102696059No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4410.10546051089758612No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC4410.10546051089758612No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4370.10450395297561255No Hit
GTGGTAGTGGTGGCACCCCATACTACGCAGAGTCCGTGAAGGGCCGGTTC4330.103547395053639No Hit
GTCCTCGGTCCTCAGGCTGTTCATTTGCAGAAACAGTGTGTTCTTGGAGGAGTCTCTGGAGACGGTGAACCGGCC4250.10163427920969184No Hit
GATCAGGACTGAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGC4240.10139513972919846No Hit
CGTGCACACTGACTGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG4220.10091686076821166No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTCAGGTGCAACTGTTGGAAT4210.10067772128771828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCC150.002441687368.3700531
ACACCGA200.00776979551.03815519
GGTATCA4350.046.1218341
GTATCAA10900.039.9325751
GAGTTTA1600.036.13041
AGTAGTT750.00271505634.1747570
CGGGTAC601.47386745E-534.02543617
TCGCTCG1151.6590631E-533.4318270
TATCAAC13000.033.2044642
ATCAACG13750.031.393313
CAACGCA13950.031.1868725
TCAACGC13950.031.1868724
GTGGTAT1209.094947E-1131.1713281
AGTTTAT1750.031.0754892
TGGTATC1101.2369128E-930.8989262
GGAGCAT450.004963428230.2195138
AGAGTAC14850.029.3037711
TACGGGT3150.029.15070215
TCTGGAG5950.029.07725570
CAGAGTA15100.028.8186110
Produced by FastQC (version 0.11.5)