FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766754
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTCCAAGGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAGACGAACTGTGG34430.4490358054865054No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27080.3531771598191858No Hit
GTAATATACGGCTGTGTCCTCAGGCTTTAGGCTGCTGATTTGCAGATAAG26600.34691700336744247No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21490.2802724211415917No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20310.26488286986438936No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20130.26253531119498563No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19160.24988457836542097No Hit
GTATATTACTGTGCGAGACTTAGTTCACACTACTACTACGGTTTGGACGT16150.21062818061594724No Hit
GTGTATTACTGTCAGCAGTATGGTCCAAGGTACACTTTTGGCCAGGGGAC15260.19902080719500648No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14830.1934127503736531No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14500.18910889281307955No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14160.18467461532642804No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13960.1820662168048683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13020.16980674375353763No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12860.16772002493628985No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT12690.1655028861929641No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC12630.1647203666364962No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG11820.15415635262417934No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11640.1518087939547756No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG11570.1508958544722297No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11550.1506350146200737No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10970.14307065890755052No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10970.14307065890755052No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10880.14189687957284866No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10830.14124477994245874No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10730.1399405806816789No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT10650.13889722127305498No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10500.13694092238188518No Hit
ATGGTACATCAAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCA10490.1368105024558072No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10490.1368105024558072No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10480.1366800825297292No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10340.13485420356463743No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAACACCAACACTGGGATCCCAACGTTTGCCCCGGGCTTCA10260.13381084415601355No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10160.13250664489523367No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10110.13185454526484375No Hit
CTCCCAGGTGCAGCTGGTTCAATCTGGGTCTGAGTTGAAGAAGCCTGGGG10030.13081118585621987No Hit
GTATGGTCCAAGGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTT9890.12898530689112805No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9870.12872446703897208No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9780.12755068770427022No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9740.12702900799995825No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9720.12676816814780228No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9360.1220730508089948No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9280.12102969140037091No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGC9270.12089927147429293No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC9230.12037759176998099No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCA9010.1175083533962653No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8740.11398701539215966No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTACAT8660.11294365598353578No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8660.11294365598353578No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT8500.11085693716628801No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA8470.11046567738805405No Hit
GTCCAGGGGCCTGTCGAACCCAATTCATAGCATAACTAGTGAACGTGTATCCAGAACCCTTGCAGGAAACCTTCA8400.10955273790550817No Hit
CATCTATGGTACATCAAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA8370.10916147812727418No Hit
GTCCAAGGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAGACGAACT8320.10850937849688426No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC8260.10772685894041634No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8200.10694433938394844No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8090.1055097201970906No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8000.1043359408623887No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA7980.10407510101023275No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7930.10342300137984282No Hit
CAGTAATATACGGCTGTGTCCTCAGGCTTTAGGCTGCTGATTTGCAGATA7890.10290132167553087No Hit
GGCCAAAAGTGTACCTTGGACCATACTGCTGACAGTAATACACTGCAAAA7850.10237964197121893No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7800.101727542340829No Hit
ACCTTGGACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT7780.10146670248867304No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7780.10146670248867304No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7760.10120586263651705No Hit
CTACTACTACGGTTTGGACGTCTGGGGCCTAGGGACCACGGTCACCGTCT7760.10120586263651705No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7740.10094502278436109No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7710.10055376300612713No Hit
GGCTTTAGGCTGCTGATTTGCAGATAAGCCGTGCTGACAGAGGTGTCCAAGGAGAAGACAAACCGTCCTGTGAAG7700.10042334308004915No Hit
GATCAACACCAACACTGGGATCCCAACGTTTGCCCCGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCT7690.10029292315397115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA200.006825724652.74216555
CGGGTAT200.00761455551.30349317
GGTATCA6500.041.5961271
GTATCAA16550.038.8775631
CGGGTCA451.0536658E-438.0025917
CCGAGTA400.00245495934.89457349
GTAGTTT750.002542500634.64230770
ATTATAC400.002718014934.1777953
TATCAAC19300.033.117432
ATCAACG20250.031.5617163
TCAACGC20450.031.2530484
CAACGCA20700.031.0427275
AACGCAG21000.030.5992626
ATACCTT450.004837253630.3822446
ACGCAGA21250.030.0784237
CGCAGAG21850.029.2524688
AGAGTAC22150.028.70196211
GCAGAGT22700.028.1571129
CGAAATT500.00735147427.88780848
CGGTACC755.8797174E-527.78984545
Produced by FastQC (version 0.11.5)