FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714022
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33390.46763264997437054No Hit
GAGTTACACTACCCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCAT31180.43668122270742354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30220.4232362588267588No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAAT26090.36539490379848244No Hit
GTATAGGGGGGTCCCGTCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATT23350.32702073605575177No Hit
ACACTACCCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGA22130.3099344277907403No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20350.28500522392867444No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAATCTGTC20080.2812238278372375No Hit
GATCTATGCTGCATCCAATTTGTATAGGGGGGTCCCGTCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA19060.2669385537140312No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAAAAG19040.2666584502998507No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18500.25909565811697677No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18220.25517421031844956No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT18090.2533535381262762No Hit
GTAATATACGGCCGTGTCGTCAGATGTCAGCCTGCTCAGCTCCATGTAGGCTGTGGTGACGGACATGTCCCTGGT17750.24859178008520746No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16540.23164552352728626No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15970.2236625762231416No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15830.22170185232387798No Hit
GTCCTGATCTATGCTGCATCCAATTTGTATAGGGGGGTCCCGTCAAGGTT15370.21525947379772611No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15340.21483931867645537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14940.20923725039284505No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14800.20727652649358144No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14430.20209461333124187No Hit
ATTATATACACTGGGTGCGACTGGCCCCTGGACAAGGGCTTGAGTGGATG13750.19257109724910437No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCATTAGCAC13580.19019021822857No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13520.18934990798602844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13180.1845881499449597No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13160.18430804653077917No Hit
GTCTGGGACAGATTTCACTGTCACCATCAGCAGTCTGCAACCTGAAGATT12970.18164706409606426No Hit
CCCCTATACAAATTGGATGCAGCATAGATCAGGACCTTAGGGGCTTTCCC12630.1768853060549955No Hit
CTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCATTAGC12500.17506463386282217No Hit
GTATATTACTGTGCGAGACGATACCTGGACCCGCCATTTTTCTACTACAT12310.17240365142810726No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12290.17212354801392674No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11780.1649809109523236No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11750.16456075583105284No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11660.1633002904672405No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAATT11620.16274008363887948No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11310.1583984807190815No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCC10830.15167599877874913No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10820.15153594707165885No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10390.14551372366677778No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCCCTTGGCCGAACGTC10370.14523362025259726No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAATTTAAAT10310.1443933100100557No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAA10240.1434129480604239No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10120.14173232757534082No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10000.14005170709025772No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9450.13234886320029354No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8870.12422586418905861No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8780.12296539882524628No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTGTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA8690.12170493346143398No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8670.12142483004725345No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC8660.1212847783401632No Hit
ACCCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC8550.11974420956217034No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8540.11960415785508009No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8430.11806358907708726No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8430.11806358907708726No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8320.11652302029909443No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8240.11540260664237235No Hit
GCCCTAACAGTGGTGGCACAAACTTTGCACAGAAGTTTCAGGGCAGGGTCACCATGACCAGGGACATGTCCGTCA8140.11400208957146978No Hit
CCTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAATTTAAATAGGTGCTA8140.11400208957146978No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8140.11400208957146978No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAATCTGTCCCAGACCCACTGCCA8010.11218141737929645No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT7970.11162121055093539No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7820.10952043494458154No Hit
GATTTCACTGTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC7730.10825996958076921No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGGCTTCTGGATACACCTTCAGCGA7640.1069995042169569No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7600.10643929738859588No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7490.10489872861060302No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7440.10419847007515175No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7290.10209769446879788No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAATTTGTATAGGGGGGTCCCG7240.10139743593334659No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7230.10125738422625634No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC7230.10125738422625634No Hit
GTCCCAGACCCACTGCCACTGAACCTTGACGGGACCCCCCTATACAAATT7180.10055712569080504No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7160.10027702227662454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT200.0073304351.79891239
GTGGTAT2200.046.5422481
TCGTATG3550.044.7877540
CTTATAC3600.043.611811
CGTATGC3650.043.60709841
TCTCCGA3650.042.99932511
CACGAGA3700.042.42122321
CCCACGA3750.041.8526819
GTATGCC3900.041.7345642
ATGCCGT3750.041.64578244
TTATACA3800.041.299092
ATCTCGT3450.040.9082637
ACGAGAC3850.040.7741622
ATCTCCG3900.040.24295810
AAAGGGG3850.038.92397768
ACACATC4050.038.7551886
CCGAGCC4150.038.63541814
CGTCTTC4000.038.3611348
CGAGCCC4100.038.27452515
TACACAT4200.038.1834955
Produced by FastQC (version 0.11.5)