FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714022
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36230.5074073347880037No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32080.4492858763455468No Hit
GAGTTACACTACCCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCAT29650.4152533115226142No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24830.34774838870510993No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAAT23570.3301018736117375No Hit
GTATAGGGGGGTCCCGTCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATT22550.3158165994885312No Hit
GTAATATACGGCCGTGTCGTCAGATGTCAGCCTGCTCAGCTCCATGTAGGCTGTGGTGACGGACATGTCCCTGGT22220.3111948931545527No Hit
ACACTACCCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGA21670.3034920492645885No Hit
GATCTATGCTGCATCCAATTTGTATAGGGGGGTCCCGTCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA20070.28108377613014723No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19850.2780026385741616No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAATCTGTC19610.27464139760399536No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAAAAG17040.2386481088817992No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16380.22940469621384216No Hit
GTCCTGATCTATGCTGCATCCAATTTGTATAGGGGGGTCCCGTCAAGGTT16340.2288444893854811No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16270.2278641274358493No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15750.22058143866715593No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14210.19901347577525622No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14210.19901347577525622No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCATTAGCAC13830.19369151090582643No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13710.19201089042074335No Hit
ATTATATACACTGGGTGCGACTGGCCCCTGGACAAGGGCTTGAGTGGATG13640.19103052847111154No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13440.18822949432930638No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12520.17534473727700267No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12470.1746444787415514No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12320.1725437031351975No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12220.17114318606429493No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12210.17100313435720468No Hit
GTATATTACTGTGCGAGACGATACCTGGACCCGCCATTTTTCTACTACAT12090.1693225138721216No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11810.16540106607359437No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11640.16302018705305998No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11540.1616196699821574No Hit
GTCTGGGACAGATTTCACTGTCACCATCAGCAGTCTGCAACCTGAAGATT11530.16147961827506715No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11490.16091941144670613No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11450.1603592046183451No Hit
CCCCTATACAAATTGGATGCAGCATAGATCAGGACCTTAGGGGCTTTCCC11370.15923879096162302No Hit
CTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCATTAGC11160.15629770511272761No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10900.15265636072838093No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAATT10580.14817470610149266No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCCCTTGGCCGAACGTC10560.14789460268731217No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAA10330.14467341342423623No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAATTTAAAT9780.13697056953427206No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9750.13655041441300128No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCC9590.13430958709955715No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8820.1235256056536073No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8750.1225452437039755No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8640.12100467492598267No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC8620.12072457151180217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8440.11820364078417751No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8280.11596281347073341No Hit
ACCCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC8240.11540260664237235No Hit
GCCCTAACAGTGGTGGCACAAACTTTGCACAGAAGTTTCAGGGCAGGGTCACCATGACCAGGGACATGTCCGTCA8230.1152625549352821No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTGTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA8180.11456229639983083No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8160.1142821929856503No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8130.11386203786437953No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8090.1133018310360185No Hit
GATTTCACTGTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC7980.11176126225802567No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7970.11162121055093539No Hit
CCTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAATTTAAATAGGTGCTA7840.10980053835876206No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7790.10910027982331076No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7690.10769976275240818No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7690.10769976275240818No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7610.10657934909568613No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT7570.1060191422673251No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7450.104338521782242No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA7350.10293800471133942No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAATCTGTCCCAGACCCACTGCCA7320.10251784959006864No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7290.10209769446879788No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7190.1006971773978953No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7180.10055712569080504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA200.00641991753.56541461
GTCGGTA200.006984889552.43524648
CGGTAGC200.0071920252.049242
CGTACCC200.007251153751.9415341
GCTATCG200.007793303651.0028911
TATCGGT306.7762035E-445.33907313
ACCGTCC306.778543E-445.33598
GTGGTAT2200.044.8584251
GTATCAA22700.038.5279541
TATCAAC24450.035.735262
AAAGTCA2450.034.6820270
ATCAACG25150.034.603043
TCAACGC25500.034.261414
CAACGCA25600.034.1299675
AACGCAG25850.033.8046236
ACGCAGA26900.032.485117
CGCAGAG27400.031.8923198
AGAGTAC27250.031.69353511
GCGAACT652.4996329E-531.50112528
GCAGAGT27900.031.19899
Produced by FastQC (version 0.11.5)