FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094871
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGGTTCAGGGAGA46040.42050616008643943No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT40940.37392533001604755No Hit
GTCTCTGGTGGCTCCATCAGTAGTGACTACTGGAGCTGGATGCGGCAGCC32720.29884799213788654No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32290.2949205888182261No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCTCCAGGACAGACA27440.2506231327708926No Hit
GTGTATTACTGTGCGAGACAGGTCTCTTATAGAAGTGGCTGGTACGAGGG25070.22897674703229878No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24880.22724138277477438No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC23950.21874723140899704No Hit
GGGTATATCTATAACAGTGGGAGCGCCAATTACAACTCCTCCCTCAAGAG23510.2147284931284142No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC23130.2112577646133654No Hit
CAGTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGGTTCAGGGA22850.20870038570754No Hit
ATCTATAACAGTGGGAGCGCCAATTACAACTCCTCCCTCAAGAGTCGAGT22700.2073303612937049No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22600.20641701168448157No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22430.20486431734880184No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20590.1880586845390918No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20030.18294392672744095No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTACCGCTGTCCCACGTCTGACAGTAATAGTCAG19860.18139123239176122No Hit
GTATTACTGTGCGAGACAGGTCTCTTATAGAAGTGGCTGGTACGAGGGCTACTTTGACTACTGGGGCCAGGGAAC19590.17892518844685812No Hit
CCCCTGTGTTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT19500.17810317379855709No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19430.17746382907210073No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19100.17444977536166362No Hit
GTACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC18890.17253174118229453No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18830.17198373141676052No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18400.16805632809710003No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGGTTTGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGCGGTA18370.167782323214333No Hit
CTCCAGGACAGACAGCCAGCATCATCTGCTCTGGAGATCATTTGGGAGAT18340.167508318331566No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTGACTACTGGAGCTGGATGCGGCAGCCCCCAGGGAAGGGA18070.1650422743866629No Hit
GTTATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGCA17940.16385491989467252No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17390.158831497043944No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16980.15508676364612817No Hit
CCCTCAGTGTCCGTGTCTCCAGGACAGACAGCCAGCATCATCTGCTCTGG16750.15298605954491443No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16710.1526207197012251No Hit
GAGTGGCTGTGTGCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT16590.151524700170157No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16560.15125069528739002No Hit
GTGTTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG16550.15115936032646768No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16500.150702685521856No Hit
GACTATTACTGTCAGACGTGGGACAGCGGTACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG16210.14805397165510822No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16090.1469579521240402No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16010.14622727243666148No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15770.1440352333745254No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15400.14065583982039892No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15040.1373677812271948No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15000.13700244138350545No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14560.13298370310292262No Hit
ACACAGGGGACTGGCCTGGCTTTTGCTGATACCAACAAACATATTTATCT14360.1311570038844759No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14210.12978697947064083No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14210.12978697947064083No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14190.12960430954879618No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14060.12841695505680578No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14030.12814295017403876No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13730.12540290134636867No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13660.1247635566199123No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13570.12394154197161128No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13530.1235762021279219No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13330.12174950290947519No Hit
GTAATAGTCAGCCTCATCCAAACCCTGGGTCCCGCTGATGGTCAGAGTGG13300.12147549802670816No Hit
CTATTACTGTCAGACGTGGGACAGCGGTACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC13190.12047081345656245No Hit
CCACAGTACCGCTGTCCCACGTCTGACAGTAATAGTCAGCCTCATCCAAA13140.12001413865195079No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG13100.11964879880826142No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12990.11864411423811573No Hit
GGACTGGAGTGGATTGGGTATATCTATAACAGTGGGAGCGCCAATTACAA12960.11837010935534871No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12440.11362069138738719No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12390.11316401658277551No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12290.11225066697355213No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCTCCAGGACAGACAGCCAGCATCAT12260.11197666209078513No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12130.11078930759879475No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12030.10987595798957138No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCCTCGTACCAGCCACTTCTA11970.10932794822403735No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11970.10932794822403735No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG11850.10823192869296931No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11840.10814059373204696No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11770.10750124900559062No Hit
GGGTTTGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGCGGTACTGTGGTATTCGGCGGAGGGACCAAG11710.10695323924005658No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11490.10494387009976518No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11350.10366518064685246No Hit
CTTCTATAAGAGACCTGTCTCGCACAGTAATACACGGCCGTGTCAGCGGC11350.10366518064685246No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG11280.10302583592039612No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGCACACAGCCACTCTGA11260.10284316599855144No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGCTTGGTATCTTGATAGATGACCAACACAGGGGA11250.1027518310376291No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11200.1022951562330174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5700.078.691370
TACCGAT11900.058.99691470
AGAGCGT200.007735579751.1007230
GTCTAAC200.00784690350.9163610
TTATACA6600.049.3734362
TATACAC6800.048.420463
CTTATAC6700.048.1606831
ATCTCGT6000.046.9262237
ACATGCG6850.046.6763427
TCGTATG6950.046.1853240
CGTATGC7000.045.91085441
GTATGCC7250.045.8215642
TACACAT7050.045.7404675
TCTCGTA6350.045.49264538
ATGCCGT7200.044.3411444
CTTGAAA7400.044.33923757
GATCTCG6350.044.28041536
ATACACA7450.044.1958584
ATCTCCG7150.044.1512510
CGCAGAT6900.044.02845432
Produced by FastQC (version 0.11.5)