FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094871
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66530.6076514950163079No Hit
GTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGGTTCAGGGAGA43170.3942930263017287No Hit
GTCTCTGGTGGCTCCATCAGTAGTGACTACTGGAGCTGGATGCGGCAGCC31210.2850564130386137No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30500.27857163081312775No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCTCCAGGACAGACA26560.242585656209727No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24950.22788072750123076No Hit
GTGTATTACTGTGCGAGACAGGTCTCTTATAGAAGTGGCTGGTACGAGGG24070.21984325094006507No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC23990.21911257125268638No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC23650.21600718258132692No Hit
GGGTATATCTATAACAGTGGGAGCGCCAATTACAACTCCTCCCTCAAGAG23520.21481982808933656No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22040.20130225387283068No Hit
ATCTATAACAGTGGGAGCGCCAATTACAACTCCTCCCTCAAGAGTCGAGT21780.19892754488884992No Hit
CAGTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGGTTCAGGGA20270.18513596578957703No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19680.17974720309515915No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19580.17883385348593578No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTACCGCTGTCCCACGTCTGACAGTAATAGTCAG19380.17700715426748906No Hit
GTATTACTGTGCGAGACAGGTCTCTTATAGAAGTGGCTGGTACGAGGGCTACTTTGACTACTGGGGCCAGGGAAC18930.1728970810259839No Hit
CCCCTGTGTTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT18690.17070504196384778No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18470.16869567282355638No Hit
GTACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC18400.16805632809710003No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17980.16422025973836185No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17820.1627589003636045No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17790.16248489548083747No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTGACTACTGGAGCTGGATGCGGCAGCCCCCAGGGAAGGGA17610.1608408661842354No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17600.16074953122331306No Hit
GTTATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGCA17440.15928817184855568No Hit
CTCCAGGACAGACAGCCAGCATCATCTGCTCTGGAGATCATTTGGGAGAT17270.15773547751287595No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16910.15444741891967181No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16900.15435608395874947No Hit
GACTATTACTGTCAGACGTGGGACAGCGGTACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG16480.15052001560001133No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16480.15052001560001133No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16410.14988067087355497No Hit
GAGTGGCTGTGTGCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT16390.1496980009517103No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGGTTTGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGCGGTA16150.1475059618895742No Hit
CCCTCAGTGTCCGTGTCTCCAGGACAGACAGCCAGCATCATCTGCTCTGG15990.1460446025148168No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15090.13782445603180649No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15090.13782445603180649No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15080.13773312107088415No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14940.1364544316179714No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14860.13572375193059275No Hit
GTGTTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG14670.13398838767306834No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14140.12914763474418448No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14080.12859962497865046No Hit
GGACTGGAGTGGATTGGGTATATCTATAACAGTGGGAGCGCCAATTACAA13950.12741227048666007No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13830.126316250955592No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13580.1240328769325336No Hit
ACACAGGGGACTGGCCTGGCTTTTGCTGATACCAACAAACATATTTATCT13560.12385020701068893No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG13390.1222975126750092No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12970.11846144431627106No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12890.11773076462889234No Hit
CCACAGTACCGCTGTCCCACGTCTGACAGTAATAGTCAGCCTCATCCAAA12880.11763942966797002No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12730.11626940525413497No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12670.11572139548860094No Hit
GTAATAGTCAGCCTCATCCAAACCCTGGGTCCCGCTGATGGTCAGAGTGG12650.11553872556675628No Hit
GGGTTTGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGCGGTACTGTGGTATTCGGCGGAGGGACCAAG12510.11426003611384354No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12470.11389469627015421No Hit
CTATTACTGTCAGACGTGGGACAGCGGTACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC12470.11389469627015421No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12350.11279867673908617No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12160.11106331248156175No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11920.10887127341942568No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11880.10850593357573633No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11870.10841459861481398No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11740.1072272441228236No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCCTCGTACCAGCCACTTCTA11720.10704457420097893No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11710.10695323924005658No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCTCCAGGACAGACAGCCAGCATCAT11630.1062225595526779No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG11500.1050352050606875No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG11280.10302583592039612No Hit
CTTCTATAAGAGACCTGTCTCGCACAGTAATACACGGCCGTGTCAGCGGC11260.10284316599855144No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11140.10174714646748338No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11050.10092513181918235No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGCACACAGCCACTCTGA11030.10074246189733767No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11010.100559791975493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)