FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463302
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57671.2447604370367493No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16390.35376493086582833No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13670.2950559246452638No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATAAA12070.2605212151037552No Hit
ACTTTATAGTTACCCTTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11400.2460598054832485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11370.24541227967934523No Hit
CTATAAAGTTGCTGACAGTAATAAGTTGCGAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC10140.21886372171931054No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9690.2091508346607612No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9600.20720825724905137No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9310.20094884114465295No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9040.1951211089095234No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8360.18044385735438226No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8330.17979633155047894No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8010.17288938964217723No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7920.17094681223046737No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7880.17008344449192966No Hit
GTGTATTACTGTGCGAGAGATTTCACCGGAACCAAGACTGGGGAAAGAAA7800.16835670901485425No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGTCTTTACTTAGCCTGGTATCAACAAAAG7760.16749334127631652No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7330.1582121380870361No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7070.15260024778654097No Hit
ACTTATTACTGTCAGCAACTTTATAGTTACCCTTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA6870.1482834090938524No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC6870.1482834090938524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6860.14806756715921796No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6720.14504578007433597No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6640.14331904459726055No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6600.14245567685872282No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6430.13878636396993754No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6240.1346853672118834No Hit
GGACATTAGTCTTTACTTAGCCTGGTATCAACAAAAGCCAGGGAAAGCCCCTAAGCTCCTGCTCTTTGGTGCGTC6100.13166358012700138No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG6070.13101605432309812No Hit
GTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATATGGGACAGAATTCACT6050.13058437045382926No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6000.1295051607806571No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5860.12648337369577511No Hit
GGATATGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTCGCAACTTATTACTGTCAGCAA5860.12648337369577511No Hit
CTCCTGTGCAGCGTCTGGATTCAGCTTCAGTGCCTATGGCTTCTACTGGGTCCGCCAGGCTCCAGGCAAGGGG5730.12367742854552755No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT5670.12238237693772097No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5670.12238237693772097No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5660.12216653500308654No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5480.11828138017966683No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTCGCAACTTATTACTGTCAGCAACTTTATAGTTAC5380.11612296083332253No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCCGTCTCCATCCTTC5320.11482790922551597No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5300.1143962253562471No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA5220.11266948987917168No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5220.11266948987917168No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC5160.11137443827136512No Hit
GTAATAAGTTGCGAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCATATCCACTGCCG5110.11029522859819296No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5060.10921601892502084No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4970.10727344151331096No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4720.10187739314745026No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4650.10036649960500926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)