FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188588
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT21721.1517169703268502No Hit
GTATAATAGTTATCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15520.8229579824803275No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC15380.8155343924321802No Hit
GAGTATTAGTGTTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGTCTCCTGATCTATAAGGCGT8710.4618533522811632No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8190.4342800178166161No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6840.3626953994951959No Hit
GTATTAGTGTTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT6310.3345918085986383No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCAGTTGCAGATCTA5810.30807898699811226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4890.25929539525314443No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4890.25929539525314443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4460.23649436867669207No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4270.22641949646849216No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4210.22323795787642906No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC4070.21581436782828176No Hit
GTTTAGAACGTGGCGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGAATTCACTCTCACCATCAGCAGC3920.20786052134812394No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT3860.20467898275606083No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3800.20149744416399773No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGTTTGGTTGGCCTGGTATCAGCAGAAA3650.1935435976838399No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC3640.1930133412518294No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG3630.19248308481981885No Hit
GTATATTACTGTGCGAGAAATCGTGCCACTGGCTGGTCATCTGACCTCTGGGGCCAGGGAACCCTGGTCACCGT3580.18983180265976626No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3550.1882410333637347No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT3420.18134769974759796No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAACAGTATAATAGTTAT3400.1802871868835769No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG3340.1771056482915138No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA3340.1771056482915138No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA3320.17604513542749275No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3090.16384923749125077No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3090.16384923749125077No Hit
GTTCTAAACTAGACGCCTTATAGATCAGGAGACTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAAA3080.16331898105924025No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC3070.1627887246272297No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3050.16172821176320867No Hit
GTACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC2990.15854667317114557No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG2970.1574861603071245No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT2920.15483487814707192No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT2920.15483487814707192No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT2840.15059282669098775No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA2830.15006257025897723No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2820.14953231382696672No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT2700.14316923664284048No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG2610.1383969287547458No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2570.13627590302670373No Hit
CAGTATAATAGTTATCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT2530.13415487729866163No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2490.13203385157061956No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC2430.12885231297855643No Hit
CACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA2330.12354974865845121No Hit
ATAGTTATCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA2320.1230194922264407No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAATG2300.12195897936241966No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2260.11983795363437759No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC2250.11930769720236707No Hit
GATCTATAAGGCGTCTAGTTTAGAACGTGGCGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGAATTCA2210.11718667147432497No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC2190.11612615861030394No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC2180.11559590217829342No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG2180.11559590217829342No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG2170.1150656457462829No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT2160.11453538931427239No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT2150.11400513288226187No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT2140.11347487645025135No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT2090.11082359429019874No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT2050.10870256856215665No Hit
GATAACTATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT1980.104990773538083No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG1970.10446051710607249No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA1970.10446051710607249No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC1930.10233949137803042No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC1910.10127897851400938No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA1890.10021846564998832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAC150.00270915866.58641
CTTGTAT150.00272058466.515782
TATAAGA150.00272058466.515782
AATGGTA609.400777E-657.1421170
TCTGGAG2400.054.28500470
TATCTCG3100.053.98622536
GTATGCC3300.053.76531242
CGTATGC3300.053.5277441
GCAATTA253.066193E-453.22673417
TCGTATG3250.053.15342340
AAAGGGG3050.052.83932568
TGCTTGA3500.052.8021755
GCCTATC3150.052.69875333
ATCTCGT3200.052.4562937
CCTATCT3200.052.0299334
CCGTCTT3400.051.7929147
TTATACA3350.051.6241842
CTATCTC3300.051.6087735
CTTGAAA3550.051.33436657
TCCGAGC3400.050.86500513
Produced by FastQC (version 0.11.5)