FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188588
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34291.8182493053640743No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC15290.8107620845440856No Hit
GTATAATAGTTATCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14820.785840032239591No Hit
GAGTATTAGTGTTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGTCTCCTGATCTATAAGGCGT8010.42473540204042676No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7710.4088277090801112No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6420.340424629350754No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5470.2900502683097546No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCAGTTGCAGATCTA5340.28315693469361786No Hit
GTATTAGTGTTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT4980.2640677031412391No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4430.2349035993806605No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4070.21581436782828176No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC3940.20892103421214497No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT3930.20839077778013448No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3660.19407385411585043No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3590.19036205909177678No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3520.18665026406770313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3430.18187795617960847No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3270.17339385326744014No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG3250.1723333404034191No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3190.16915180181135597No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT3190.16915180181135597No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG3170.16809128894733494No Hit
GTATATTACTGTGCGAGAAATCGTGCCACTGGCTGGTCATCTGACCTCTGGGGCCAGGGAACCCTGGTCACCGT3130.16597026321929284No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3120.16544000678728232No Hit
GTTTAGAACGTGGCGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGAATTCACTCTCACCATCAGCAGC3110.1649097503552718No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA2970.1574861603071245No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT2930.15536513457908244No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2920.15483487814707192No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC2900.15377436528305088No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAACAGTATAATAGTTAT2890.15324410885104037No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT2870.1521835959870193No Hit
GAGTGAATTCTGTCCCAGAGCCACTGCCGCTGAACCTTGATGGGACGCCA2810.1490020573949562No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA2780.14741128809892465No Hit
GTACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC2770.14688103166691413No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTGTTTGGTTGGCCTGGTATCAGCAGAAA2760.14635077523490359No Hit
GTTCTAAACTAGACGCCTTATAGATCAGGAGACTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAAA2710.143699493074851No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT2670.14157846734680893No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA2670.14157846734680893No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT2640.13998769805077735No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC2460.130443082274588No Hit
CAGTATAATAGTTATCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT2410.1277918001145354No Hit
ATAGTTATCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA2350.12461026152247227No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2340.12408000509046176No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAATG2340.12408000509046176No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG2310.12248923579443018No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2260.11983795363437759No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2250.11930769720236707No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA2230.11824718433834604No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2230.11824718433834604No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2160.11453538931427239No Hit
CACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA2150.11400513288226187No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2110.11188410715421977No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA2050.10870256856215665No Hit
GATAACTATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT2040.10817231213014614No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC2030.10764205569813562No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT2020.1071117992661251No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT2020.1071117992661251No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC2010.10658154283411458No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC1980.104990773538083No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGC1980.104990773538083No Hit
GATCTATAAGGCGTCTAGTTTAGAACGTGGCGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGAATTCA1970.10446051710607249No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT1960.10393026067406197No Hit
ACTTATTACTGCCAACAGTATAATAGTTATCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA1940.10286974781004093No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA1930.10233949137803042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGTA150.002554633267.5797832
CTTGTCA253.0768698E-453.189282
ATAGTAA200.007894441550.82182333
TTGTCAG200.00851061949.8649563
AATGGTA702.2210332E-549.41678670
TCTGGAG1700.048.8354170
CGGGTAG307.541443E-444.34792717
GACAGAC406.167394E-541.5982551
TACCACT350.001613987737.9923484
TCAGTTG1100.037.2939536
GACAGGA451.2343704E-436.9762271
TGAATTC4300.036.8588665
AATTCTG4200.036.0167667
GTATCAA5400.035.7436871
GAATTCT4200.035.5655966
ATCTAGT1201.8189894E-1235.15437346
TTCTGTC5650.035.02463569
ACGGGTA953.2196112E-1035.0115216
ATTGTCT1301.8189894E-1234.59413564
AGAGTGA4400.034.4480161
Produced by FastQC (version 0.11.5)