FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150755
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGAGAGTCTGAGTGCTTGGGTGTTCGG61020.5302605680618377No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCAATGATGGCCAGGGAGG60980.5299129701804467No Hit
GTCCTGGGCCCAGTCTGTCCTGATTCAGCCACCCTCAGCGTCTGGGACCC50900.4423183040699367No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47500.41277248415170903No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGCACTCAGACTCTCATCCCATG41460.36028520406168124No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGATTCAGCCACCCTCAGCGTCT41240.35837341571403125No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGCACTCAGA37010.3216149397569422No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCCGCTATCTGCCTTCCAG36460.31683546888781716No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35200.30588613562400335No Hit
GTCCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC32060.2785997019348167No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC31040.2697359559593484No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30600.26591237926404837No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT29820.2591342205769256No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29170.25348575500432324No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGATTCAGCCAC27140.23584516252373441No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26630.23141328953600027No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24530.21316440076297732No Hit
GGATGAGAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC24350.21160021029671827No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA24180.21012291930080687No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24090.2093408240676773No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23570.20482205160959546No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCCGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22990.1997818823294272No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22810.19821769186316812No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22440.19500241146030217No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21900.19030984006152482No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21640.1880504538324839No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG20760.18040330044188382No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19590.1702360624111996No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19250.16728148041937682No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19210.16693388253798594No Hit
CTCTTGTTCTGGAAGCCACTCCAATATCGGAAATAATTATGTCTCCTGGTACCAGCAATTCCCAGGAACGGCCCC19090.16589108889381318No Hit
GTCTCTGGTGGCTCCATTAGTCCTTATTACTGGAGTTGGATCCGGCAGCC18910.16432689842755407No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG18900.16423999895720634No Hit
GTATTATTGTGTGAGAGCGATTTCGGATAGAAGTGGTTTTTATTCCGGGA18530.16102471855434042No Hit
CTCTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG18450.1603295227915586No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18200.15815703603286538No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGCACTCAGAC18000.15641904662591083No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17740.15415966039686987No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17630.15320376622304485No Hit
TCATTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG17530.1523347715195676No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17520.15224787204921986No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17350.15077058105330848No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAAAATTCTGGAGGGGC17300.15033608370156984No Hit
CTCCAATATCGGAAATAATTATGTCTCCTGGTACCAGCAATTCCCAGGAA16760.14564351230279252No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCCCAGCCTCAGGGAGACCTGGTTCTTGGACATGTCGA16640.14460071865861976No Hit
CCCAAGCACTCAGACTCTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAATGATGG16440.1428627292516652No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAAAATTC16300.141646136666797No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15780.13712736420871516No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15620.1357369726831515No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15450.13425968168724012No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15180.13191339598785146No Hit
GTCATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG14840.1289588139960287No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14590.1267863272373355No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCATTGGGCTCCGGTCCGAGGATG14460.12565663412281503No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14320.12444004153794683No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT13990.1215723590164718No Hit
GTTGAGAACCTATGAAAATTCTGGAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13880.12061646484264679No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13860.12044266590195132No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13770.11966057066882178No Hit
CTCAGACTCTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGA13760.11957367119847405No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13700.11905227437638767No Hit
CCCCTGAGGGCCGGTGATTATGACTAAACATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGGAGA13680.11887847543569222No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAG13560.11783568179151947No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13480.11714048602873765No Hit
GAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT13440.11679288814734676No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC13360.11609769238456492No Hit
GTTTGGTCCCTCCGCCGAACACCCAAGCACTCAGACTCTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT13220.11488109979969673No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13090.11375140668517625No Hit
GCATGGGATGAGAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG12740.11070992522300577No Hit
GTGCAGCATGGGATGAGAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGACC12720.11053612628231031No Hit
CTCCACTCCCGCCTTGACGGGGCCGCTATCTGCCTTCCAGGCCACTGTCA12720.11053612628231031No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12680.11018852840091939No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGATTCAGCCACCCTCAGCGTCTGGGA12670.11010162893057168No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12620.10966713157883302No Hit
AGTCTGTCCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG12600.10949333263813757No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCC12390.10766844376083529No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGAGAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGAC12310.10697324799805345No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGC12250.10645185117596709No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCATTGGGCTCCGGT12210.10610425329457616No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12130.10540905753179435No Hit
GTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGGAGACATAATTATTT12090.10506145965040344No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC12010.10436626388762162No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12000.10427936441727388No Hit
GGCCCAGTCTGTCCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC11960.10393176653588296No Hit
GCCCCCAAACTCCTCATGTTTAGTCATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT11950.10384486706553524No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGATTCAGCCACCCTCAG11940.1037579675951875No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGGTG11770.10228067659927612No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11720.10184617924753749No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11690.1015854808364943No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11520.10010818984058291No Hit
GGGTTGAGAACCTATGAAAATTCTGGAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11510.1000212903702352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5550.084.30961670
TACGTAT503.7333812E-666.8454970
CCGTCGT451.6689683E-646.73444447
TCTCGTA5550.046.35838338
CGTATGC6100.045.85702541
GGTATCA10100.045.7398261
GCGAATC5850.045.3332833
TAAGGCG6500.045.2813729
TCGTATG6100.045.20836640
GTGGTAT2700.044.9608041
ATCTCGT5750.044.63143537
CTTATAC6650.044.6227531
GGCGAAT6500.044.41475732
CGAATCT5850.044.2232234
ACTAAGG6650.044.17905427
ATGCCGT6150.044.1593944
CGAGACT6650.044.10225723
GTCGTCT404.4234483E-544.03241349
AATCTCG5900.043.9874436
GAATCTC5900.043.91783535
Produced by FastQC (version 0.11.5)