FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150755
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60330.5242645046078444No Hit
GATTATTACTGTGCAGCATGGGATGAGAGTCTGAGTGCTTGGGTGTTCGG58430.507753605241776No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCAATGATGGCCAGGGAGG55970.4863763355362349No Hit
GTCCTGGGCCCAGTCTGTCCTGATTCAGCCACCCTCAGCGTCTGGGACCC48820.4242432142376092No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44970.39078691815373384No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGATTCAGCCACCCTCAGCGTCT40210.3494227702682152No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGCACTCAGACTCTCATCCCATG39030.3391686327671833No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCCGCTATCTGCCTTCCAG35060.3046695430391352No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32730.28442196644811446No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGCACTCAGA32690.2840743685667236No Hit
GTCCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC32040.27842590299412123No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30330.2635660935646597No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27770.24131982915564126No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27550.23940804080799125No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC27390.2380176492824276No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA26520.23045739536217527No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGATTCAGCCAC26040.22628622078548433No Hit
GGATGAGAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC25810.22428753296748655No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24720.2148154906995842No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23020.2000425807404704No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22960.19952118391838403No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22500.19552380828238852No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21070.18309718402266337No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCCGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20990.18240198825988155No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20630.17927360732736333No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20570.17875221050527698No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG20520.1783177131535383No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19600.17032296188154733No Hit
CTCTTGTTCTGGAAGCCACTCCAATATCGGAAATAATTATGTCTCCTGGTACCAGCAATTCCCAGGAACGGCCCC18910.16432689842755407No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18480.16059022120260175No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18090.15720114185904038No Hit
GTCTCTGGTGGCTCCATTAGTCCTTATTACTGGAGTTGGATCCGGCAGCC18000.15641904662591083No Hit
TCATTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG17810.154767956689304No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG17690.15372516304513123No Hit
CTCTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG17610.1530299672823494No Hit
CTCCAATATCGGAAATAATTATGTCTCCTGGTACCAGCAATTCCCAGGAA17520.15224787204921986No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17490.15198717363817665No Hit
GTATTATTGTGTGAGAGCGATTTCGGATAGAAGTGGTTTTTATTCCGGGA17300.15033608370156984No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17180.1492932900573971No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16800.14599111018418343No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAAAATTC16630.14451381918827205No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAAAATTCTGGAGGGGC16370.1422544329592311No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16060.13956054937845153No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAGCACTCAGAC16050.13947364990810382No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15520.1348679779796742No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14700.1277422214111605No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14650.12730772405942187No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCCCAGCCTCAGGGAGACCTGGTTCTTGGACATGTCGA14420.12530903624142412No Hit
CCCAAGCACTCAGACTCTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAATGATGG14370.12487453888968547No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14130.12278895160134No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAAG14090.12244135371994908No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13940.12113786166473314No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13810.12000816855021268No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT13570.11792258126186721No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13550.11774878232117177No Hit
GTTGAGAACCTATGAAAATTCTGGAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13460.1169666870880422No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13440.11679288814734676No Hit
GTCATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13370.11618459185491264No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCATTGGGCTCCGGTCCGAGGATG13320.115750094503174No Hit
CCCCTGAGGGCCGGTGATTATGACTAAACATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGGAGA13200.11470730085900126No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCC13100.11383830615552398No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13060.11349070827413306No Hit
GAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT13020.11314311039274216No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12870.11183961833752623No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12630.10975403104918076No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGATTCAGCCACCCTCAG12580.10931953369744211No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12410.10784224270153073No Hit
GCATGGGATGAGAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG12370.10749464482013982No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC12220.1061911527649239No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12180.10584355488353298No Hit
GGGTCACCATCTCTTGTTCTGGAAGCCACTCCAATATCGGAAATAATTAT12090.10506145965040344No Hit
CTCCACTCCCGCCTTGACGGGGCCGCTATCTGCCTTCCAGGCCACTGTCA12090.10506145965040344No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12080.1049745601800557No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGC12020.10445316335796932No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12000.10427936441727388No Hit
GTTTGGTCCCTCCGCCGAACACCCAAGCACTCAGACTCTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT11990.10419246494692615No Hit
GGGTTGAGAACCTATGAAAATTCTGGAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11980.10410556547657843No Hit
GTGCAGCATGGGATGAGAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGACC11890.10332347024344887No Hit
CTCAGACTCTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGA11830.1028020734213625No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11780.10236757606962385No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11760.10219377712892841No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGAGAGTCTGAGTGCTTGGGTGTTCGGCGGAGGGAC11670.10141168189579884No Hit
GTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGGAGACATAATTATTT11660.10132478242545111No Hit
GCCCCCAAACTCCTCATGTTTAGTCATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT11660.10132478242545111No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG11620.10097718454406021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.046.7326241
ACGTTCG306.0161314E-446.44936444
TGGTATC2350.044.3894962
GTGGTAT2450.043.9836461
CAACTGG6250.040.0547670
GCCAGAG21900.038.10384870
GTATCAA24650.036.612151
TATCAAC26700.033.9021642
TGGGTCG552.6883848E-432.40629653
ATCAACG28400.031.8672683
AATGGTA3500.031.78949270
TCAACGC28750.031.4793194
CAACGCA28750.031.3636575
TCGAATG450.004204099531.2672848
AACGCAG28800.031.196456
ACGCAGA30200.029.862987
CGCAGAG30700.029.3766088
AGAGTAC30950.029.24804711
CGGGATA2450.028.85171917
GCAGAGT31550.028.5851679
Produced by FastQC (version 0.11.5)