FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509352
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21650.4250498672823509No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC18990.37282665033218676No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18420.36163596098572304No Hit
GGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC16660.32708225352997533No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15820.3105907113351867No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATTGCCTGTGCCCCAGGTCTGACAGTAATAGTCAG15260.2995963498719942No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT15190.2982220546890952No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14910.2927248739574989No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14580.28624605380954626No Hit
GATTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT14190.2785892663619658No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13560.26622060971587425No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13500.26504264241624653No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13160.25836749438502254No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12940.2540482809530541No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT12890.2530666415366976No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12470.24482087043930326No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12430.2440355589062181No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12090.2373604108749941No Hit
GACTATTACTGTCAGACCTGGGGCACAGGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG11820.2320595580266692No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11640.22852565612778591No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC11300.22185050809656193No Hit
CTGTTAATCTTCATCAAGTTCCGAGGGCCCTTTTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC10870.21340840911589629No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10660.20928552356719912No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10610.20830388415084264No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT10420.20457365436868805No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG10410.20437732648541676No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG10390.20398467071887416No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10330.20280670341924642No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA10270.20162873611961865No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10240.20103975246980477No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC10000.19632788327129375No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9990.1961315553880224No Hit
CTATTACTGTCAGACCTGGGGCACAGGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC9770.19181234195605396No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9720.1908307025396975No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGC9460.18572617757464388No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8920.175124471877994No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8830.17335752092855236No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC8590.1686456517300413No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8560.16805666808022743No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG8130.1596145690995618No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC8100.1590255854497479No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8060.15824027391666273No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGT7890.15490269990105077No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC7850.15411738836796557No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA7710.15136879800216746No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT7630.1497981749359971No Hit
GGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT7630.1497981749359971No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC7610.14940551916945452No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7610.14940551916945452No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7580.14881653551964064No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7460.14646060092038513No Hit
CTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT7310.1435156826713157No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7300.14331935478804442No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7110.13958912500588982No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC6970.13684053464009174No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG6910.13566256734046395No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACAGGCAATGTGGTATTCGGCGGAGGGACCAAG6870.1348772558073788No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6780.13311030485793712No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATTGCCTGTGCCCCAGGTCTGACAGTAATAGTCAGC6670.13095069814195293No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6650.13055804237541033No Hit
CCACATTGCCTGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCA6620.12996905872559644No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGC6580.12918374719251127No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC6290.12349023857764374No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6080.11936735302894659No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6050.1187783693791327No Hit
GTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTG6010.11799305784604754No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATTGC5990.11760040207950492No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCT5970.11720774631296235No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5810.11406650018062166No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5750.1128885328809939No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5690.11171056558136613No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACT5590.1097472867486532No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5570.1093546309821106No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5550.10896197521556802No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG5550.10896197521556802No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA5540.10876564733229672No Hit
GTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG5520.10837299156575414No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5500.10798033579921154No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCGGAGGGC5420.1064097127330412No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5350.10503541755014215No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTG5350.10503541755014215No Hit
GGATGAGGCTGACTATTACTGTCAGACCTGGGGCACAGGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGT5300.10405377813378566No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGGCACAGC5280.10366112236724308No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC5190.10189417141780145No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG5150.10110885988471627No Hit
CTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAG5100.10012722046835981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2400.073.65224570
CGCGTAA451.638373E-460.4326170
GTATGCC2200.051.92697542
CGTACTA2200.050.9147229
ACCGTAC2200.050.85963427
TCTCGTA2050.050.2717838
TTATACA2300.050.0290532
CTAGATC2050.049.86928633
GACCGTA2250.049.7196426
CGTATGC2300.049.5945841
ATCTCGT2100.048.97235537
TAGATCT2100.048.7493834
GTACTAG2300.048.7442230
TCGTATG2350.048.45194240
ATGCCGT2400.047.8109644
TGGTATC1350.047.6311452
AGACCGT2400.046.580125
CTTATAC2400.046.543511
TATACAC2400.046.5343743
ACACATC2500.046.0357676
Produced by FastQC (version 0.11.5)