FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509352
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25880.5080965619061082No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21680.4256388509321648No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19020.37341563398200067No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC17110.33591700827718357No Hit
GGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC16820.33022349966231607No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15030.29508080855675445No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14670.2880130047589879No Hit
GATTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT14240.2795709057783223No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATTGCCTGTGCCCCAGGTCTGACAGTAATAGTCAG14140.27760762694560936No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13400.2630793635835336No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT12820.25169234635379856No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12780.25090703482071336No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12710.24953273963781433No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11900.23363018109283953No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11640.22852565612778591No Hit
GACTATTACTGTCAGACCTGGGGCACAGGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG11540.22656237729507295No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11030.21654965524823697No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10990.2157643437151518No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10880.21360473699916757No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10620.20850021203411392No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC10600.20810755626757133No Hit
CTGTTAATCTTCATCAAGTTCCGAGGGCCCTTTTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC10520.206536933201401No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10390.20398467071887416No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10090.19809483422073537No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10030.19691686692110763No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG9840.193186637138953No Hit
CTATTACTGTCAGACCTGGGGCACAGGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC9750.19141968618951138No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9640.18926007947352713No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGC9540.1872968006408142No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA9330.18317391509211706No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8930.1753207997612653No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGT8920.175124471877994No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG8890.17453548822818013No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8890.17453548822818013No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC8830.17335752092855236No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT8660.17001994691294037No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC8330.16354112676498767No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC8160.16020355274937567No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8150.16000722486610439No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8110.15922191333301922No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8030.15765129026684885No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA8010.15725863450030628No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7980.1566696508504924No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7860.15431371625123685No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7650.1501908307025397No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7620.14960184705272583No Hit
CTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT7600.1492091912861832No Hit
GGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT7460.14646060092038513No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG7400.14528263362075736No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACAGGCAATGTGGTATTCGGCGGAGGGACCAAG7350.14430099420440087No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC7190.1411597480720602No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT6910.13566256734046395No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGC6900.13546623945719266No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC6890.13526991157392138No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC6750.13252132120812327No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCT6630.13016538660886773No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6590.12938007507578256No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6480.12722046835979833No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC6440.12643515682671316No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6360.12486453376054281No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG6230.12231227127801599No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATTGCCTGTGCCCCAGGTCTGACAGTAATAGTCAGC6150.12074164821184565No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6020.11818938572931884No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATTGC5900.1158334511300633No Hit
GTCTCTCTCCCAGCTTGTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTG5840.11465548383043553No Hit
CCACATTGCCTGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCA5820.11426282806389296No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5790.11367384441407907No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC5760.11308486076426517No Hit
GTGTTGACTCAATCGCCCTCCGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG5740.11269220499772259No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5700.11190689346463742No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5660.11112158193155224No Hit
GTATATTACTGTGCGGCCCAGGTGACTACGGTTGACTACTGGGGCCAGGG5640.11072892616500965No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC5540.10876564733229672No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTG5510.10817666368248284No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG5420.1064097127330412No Hit
GGATGAGGCTGACTATTACTGTCAGACCTGGGGCACAGGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGT5120.10051987623490238No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5110.10032354835163108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1150.049.9420972
GGTATCA4350.047.394381
GTATCAA14400.043.420571
TCTGGAG3100.043.38960370
GGTACGG350.001494014438.6069074
TATCAAC16250.037.838482
CTTCCGG750.001665054537.7565770
AACGCAG16600.036.640846
ACGGGCC1200.036.61769516
ATCAACG17250.035.6414533
TCAACGC17300.035.1479074
CAACGCA17450.034.8526235
ACGCAGA17500.034.5633627
CGCAGAG17550.034.272358
TAACGGC501.948505E-434.22791736
GTAGGTC400.002862030433.8175620
ACAGGTA701.1418233E-633.7976341
AGAGTAC17900.033.79099311
AGGTACG400.0028772333.7810443
CAGAGTA18050.033.3229810
Produced by FastQC (version 0.11.5)