FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251800
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54190.43289662885444957No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46400.37066624061351655No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA40740.32545135005591946No Hit
GTATAATAACTGGTTTGTGATCAGCTTCGGCCAAGGGACACGACTGGAGATCAAACGAACTGTGGCTGCACCAT40120.32049848218565263No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA37210.2972519571816584No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34180.27304681258987057No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32560.2601054481546573No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32540.25994567822335835No Hit
CATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA29840.23837673749800287No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATT29750.2376577728071577No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGT29660.23693880811631254No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29320.2342227192842307No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29300.2340629493529318No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27690.22120146988336792No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAG27640.22080204505512063No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAGTGGCCCTGGT27450.2192842307077808No Hit
GTGTATTACTGTGCGAGAGATTCCGCAGGGGTTGATAATGATTTTGACTC26970.21544975235660652No Hit
CTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCT26690.21321297331842148No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25530.20394631730308357No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24780.1979549448793737No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24270.19388081163125098No Hit
CTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT24010.1918038025243649No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23040.18405496085636683No Hit
CCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGGC22860.18261703147467648No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC22430.17918197795174948No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21340.1704745166959578No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21090.16847739255472122No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21070.16831762262342229No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCCCTTGGCCGAAGCTGATCACAAACCAGTTATTATACTG20600.16456302923789742No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20460.16344463971880493No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19800.15817223198594024No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGTTTGTGATCAGCTTCGGCCA19610.1566544176386004No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19350.15457740853171434No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT18970.15154177983703465No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18760.14986419555839592No Hit
GTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATCCA17860.14267454864994408No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17640.14091707940565584No Hit
GTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17310.13828087553922352No Hit
GGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGT17180.13724237098578046No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATTTTGCAGTTTA17180.13724237098578046No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16520.13196996325291582No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16490.1317303083559674No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCA16380.13085157373382328No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16260.12989295414602972No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16120.1287745646269372No Hit
TCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16010.1278958300047931No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCC15930.12725675027959737No Hit
GGATATCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGG15600.12462054641316504No Hit
CTCCTGTGCAGCGTCTGGATTCACGTTTAGAAGTTATGGCATGCACTGGG15410.12310273206582521No Hit
GTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTG15400.12302284710017575No Hit
CCCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCA14920.11918836874900143No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTC14830.11846940405815627No Hit
CTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCT14510.11591308515737338No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC14490.11575331522607445No Hit
GGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14410.11511423550087874No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14240.11375619108483784No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14150.11303722639399265No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13900.11104010225275604No Hit
CAGTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATA13620.10880332321457102No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13580.10848378335197315No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13570.10840389838632371No Hit
GTCTGCGTCTCCAGGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTA13560.10832401342067424No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13430.10728550886723118No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC13230.10568780955424188No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13200.1054481546572935No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12970.10361080044735581No Hit
GTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCGAGAACACACTGTATCTGCAAATGAA12950.10345103051605686No Hit
ACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTT12950.10345103051605686No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12940.10337114555040743No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12880.10289183575651062No Hit
GTATTACTGTGCGAGAGATTCCGCAGGGGTTGATAATGATTTTGACTCCTGGGGCCAGGGAACACTGGTCATCGT12870.10281195079086115No Hit
CAGTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12840.10257229589391277No Hit
CCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGGGTCA12770.1020131011343665No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12680.10129413644352132No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTC12680.10129413644352132No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12660.1011343665122224No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT12650.10105448154657294No Hit
GTCGTGTCCCTTGGCCGAAGCTGATCACAAACCAGTTATTATACTGCTGA12610.10073494168397507No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12550.10025563189007829No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12530.10009586195877936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG200.007776854651.0324175
TCGTATG2450.045.00267840
CGTATGC2650.044.2603641
GGTATCA11100.041.7057951
ACTAAGG3100.040.65678427
TCTGGAG12900.040.23459670
GACTAAG3050.040.19358426
CACGAGA3150.039.9730721
GCGAATC2500.039.6694933
TAAGGCG3150.038.96277629
TCTCCGA3150.038.88028711
GGCGAAT3000.038.72334332
CGAATCT2400.038.5096834
ATCTCCG3200.038.27278510
ACGAGAC3250.037.6990322
GTCATCG11450.037.29667770
GAGACTA3300.037.13673824
ATCTCGT2500.037.0556837
ATGCCGT3150.036.3281144
AATCTCG2550.036.30130836
Produced by FastQC (version 0.11.5)