FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251800
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55460.4430420194919316No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42050.33591628055599937No Hit
GTATAATAACTGGTTTGTGATCAGCTTCGGCCAAGGGACACGACTGGAGATCAAACGAACTGTGGCTGCACCAT37780.3018054002236779No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37250.2975714970442563No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA34120.2725675027959738No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33410.2668956702348618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32550.2600255631890078No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29450.23526122383767376No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29190.23318421473078765No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATT29000.23166640038344782No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28700.2292698514139639No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGT28460.22735261223837672No Hit
GTGTATTACTGTGCGAGAGATTCCGCAGGGGTTGATAATGATTTTGACTC27650.2208819300207701No Hit
CATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA27060.21616871704745166No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26860.21457101773446235No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAG25680.20514459178782554No Hit
CTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCT25550.20410608723438248No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAGTGGCCCTGGT24900.19891356446716726No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23850.1905256430739735No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23350.18653139479150024No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23300.18613196996325293No Hit
CTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT22290.17806358843265696No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21580.17239175587154498No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC21450.17135325131810195No Hit
CCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGGC21350.1705544016616073No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21140.16887681738296853No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20610.1646429142035469No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCCCTTGGCCGAAGCTGATCACAAACCAGTTATTATACTG20570.164323374340949No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20410.1630452148905576No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20350.1625659050966608No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19930.1592107365393833No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19900.1589710816424349No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19140.15289982425307555No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT19000.15178143473398306No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGTTTGTGATCAGCTTCGGCCA18710.14946477073014858No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18500.14778718645150984No Hit
GTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17510.13987857485221283No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17240.13772168077967728No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17130.13684294615753315No Hit
GTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATCCA17120.1367630611918837No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATTTTGCAGTTTA17060.1362837513979869No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCA17040.13612398146668797No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16750.1338073174628535No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTC15570.12438089151621665No Hit
GGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGT15460.12350215689407253No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCC15070.12038664323374342No Hit
CTCCTGTGCAGCGTCTGGATTCACGTTTAGAAGTTATGGCATGCACTGGG14910.11910848378335198No Hit
GGAGAAATAGTGGTGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCAGG14800.11822974916120786No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14670.11719124460776481No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14630.11687170474516696No Hit
GGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14570.11639239495127016No Hit
GTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTG14460.11551366032912606No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14400.11503435053522926No Hit
GGATATCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGG14300.11423550087873462No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14270.11399584598178623No Hit
TCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14240.11375619108483784No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14110.1127176865313948No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13890.11096021728710656No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13790.11016136763061192No Hit
CCCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCA13470.10760504872982905No Hit
GTCTGCGTCTCCAGGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTA13450.10744527879853012No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13380.10688608403898386No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13320.10640677424508707No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13200.1054481546572935No Hit
CAGTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATA13140.10496884486339671No Hit
GTCGTGTCCCTTGGCCGAAGCTGATCACAAACCAGTTATTATACTGCTGA13060.10432976513820098No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12820.10241252596261384No Hit
CTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCT12690.10137402140917079No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC12590.10057517175267616No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12530.10009586195877936No Hit
CACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG12520.10001597699312989No Hit
GTATTACTGTGCGAGAGATTCCGCAGGGGTTGATAATGATTTTGACTCCTGGGGCCAGGGAACACTGGTCATCGT12520.10001597699312989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT200.0078469350.9167416
GGTATCA10450.046.147671
TAACGGC451.940938E-645.7217536
AATGGTA4150.044.24833770
TTAACGG551.5529804E-743.6029935
TCTGGAG12550.039.5603870
GTCATCG10500.038.8636270
GTATCAA26350.038.020611
CGCGGCA400.002491443234.79150845
CACGACG501.9199235E-434.3190337
ATCAACG31700.031.0272733
TCAACGC31950.030.7844964
AATACGT2100.030.68837410
CAACGCA32550.030.2182455
AACGCAG32700.030.1845596
GAATACG2150.029.9746939
AACGGCC703.9919476E-529.41631137
ATACGTA2200.029.2934511
CTAGAGA2550.029.260884
CTCTGGA12800.028.72350569
Produced by FastQC (version 0.11.5)