FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294436
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT24590.8351560271162495No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11260.38242606203045826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9220.3131410561208548No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7050.23944082924642365No Hit
GAGTTACAGTGATTATCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT6620.22483663682430136No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6360.21600619489464604No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6360.21600619489464604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6060.20581722343735143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5880.19970384056297463No Hit
CTACCTACACTATGCAGACTCAGTGAGGGGCCGATTCACCATCTCCAGAGACAACTCCAAGAATTCACTGTAT5810.1973264138896059No Hit
GTGCATCCACCTTGCAAAGTGGGGTCCCATCACGGTTCAGTGGCAGTGGA5800.19698678150769605No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5780.19630751674387642No Hit
GTAGGAGACAGAGTCACCATTACCTGCCGGGCAAGTCAGACCATCATGAACTATTTAAATTGGCATCAGCAGAAA5510.18713744243231129No Hit
CTGTAACTCTGTTGACAGTAGTAAACTGCAAAATCTTCAGGTTGCAGATTGTTGATGGTGAGAGTGAAATCTGTC5450.18509964814085234No Hit
ATAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA5280.17932589764838539No Hit
GTTTACTACTGTCAACAGAGTTACAGTGATTATCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAGACGAA5100.17321251477400862No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4990.1694765585730006No Hit
GTGTAGGTAGAGACTACTACTACTAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT4970.16879729380918093No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4940.16777839666345148No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4840.16438207284435327No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATTACCTGCCGGGCAAGTCAGACCATCATGAA4730.16064611664334524No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG4730.16064611664334524No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAATCTGCAACCTGAAGATTTTGCAGTTTA4670.15860832235188632No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4640.15758942520615687No Hit
ATCATGAACTATTTAAATTGGCATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTACGGTGCATCCAC4630.15724979282424703No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4520.153513836623239No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4490.15249493947750956No Hit
GTAGTAAACTGCAAAATCTTCAGGTTGCAGATTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA4440.15079677756796042No Hit
GATCTACGGTGCATCCACCTTGCAAAGTGGGGTCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA4410.14977788042223097No Hit
GTGGATGCACCGTAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTG4250.14434376231167384No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4190.14230596802021492No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC4190.14230596802021492No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4110.13958890896493634No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCAAAAGTG3960.13449442323628905No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCATCCAGATGACCCAGTC3870.13143773179910065No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCAAAAGTGAGATAATCA3850.130758467035281No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCAT3730.12668287845236317No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3690.12532434892472388No Hit
CCATTACCTGCCGGGCAAGTCAGACCATCATGAACTATTTAAATTGGCAT3680.12498471654281405No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3670.12464508416090422No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3620.12294692225135515No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3620.12294692225135515No Hit
GATTTCACTCTCACCATCAACAATCTGCAACCTGAAGATTTTGCAGTTTA3550.1205694955779864No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3510.11921096605034709No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT3490.11853170128652746No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3480.11819206890461764No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC3340.11343721555788017No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT3330.11309758317597032No Hit
GTCTATTATTGTGCGAGCCCCACTGGAAGCACGGTTCACTGGGGCCAGGG3300.11207868603024086No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3290.11173905364833105No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3260.11072015650260159No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG3140.10664456791968373No Hit
CAATAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA3120.1059653031558641No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT3080.10460677362822482No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3080.10460677362822482No Hit
AGTCAGACCATCATGAACTATTTAAATTGGCATCAGCAGAAACCAGGGAA3040.10324824410058553No Hit
GTAGATATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG3010.10222934695485607No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG3010.10222934695485607No Hit
GTGCCAGATGTGGCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATTA3010.10222934695485607No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT2960.10053118504530696No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2950.10019155266339713No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC2950.10019155266339713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)