FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294436
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40801.385700118192069No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10870.3691803991359752No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7350.2496298007037183No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6680.22687443111576033No Hit
GAGTTACAGTGATTATCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT6620.22483663682430136No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6320.21464766536700675No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5800.19698678150769605No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5780.19630751674387642No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5710.19393009007050768No Hit
CTACCTACACTATGCAGACTCAGTGAGGGGCCGATTCACCATCTCCAGAGACAACTCCAAGAATTCACTGTAT5660.19223192816095858No Hit
GTGCATCCACCTTGCAAAGTGGGGTCCCATCACGGTTCAGTGGCAGTGGA5430.1844203833770327No Hit
CTGTAACTCTGTTGACAGTAGTAAACTGCAAAATCTTCAGGTTGCAGATTGTTGATGGTGAGAGTGAAATCTGTC5190.17626920621119702No Hit
GTGTAGGTAGAGACTACTACTACTAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT4980.16913692619109075No Hit
GTAGGAGACAGAGTCACCATTACCTGCCGGGCAAGTCAGACCATCATGAACTATTTAAATTGGCATCAGCAGAAA4770.16200464617098453No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4660.1582686899699765No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4580.15555163091469792No Hit
ATAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA4510.1531742042413292No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4510.1531742042413292No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4440.15079677756796042No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATTACCTGCCGGGCAAGTCAGACCATCATGAA4400.14943824804032116No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4380.14875898327650153No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4350.14774008613077205No Hit
ATCATGAACTATTTAAATTGGCATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTACGGTGCATCCAC4310.14638155660313276No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG4300.14604192422122295No Hit
GTAGTAAACTGCAAAATCTTCAGGTTGCAGATTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA4240.14400412992976402No Hit
GTTTACTACTGTCAACAGAGTTACAGTGATTATCTCACTTTTGGCGGAGGGACCAAGGTGGAGATCAGACGAA4240.14400412992976402No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAATCTGCAACCTGAAGATTTTGCAGTTTA4220.1433248651659444No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4160.14128707087448544No Hit
GTGGATGCACCGTAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTG4050.13755111467347744No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3960.13449442323628905No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3920.13313589370864976No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3890.1321169965629203No Hit
GATCTACGGTGCATCCACCTTGCAAAGTGGGGTCCCATCACGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA3850.130758467035281No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3840.13041883465337117No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCATCCAGATGACCCAGTC3810.12939993750764173No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3690.12532434892472388No Hit
CCATTACCTGCCGGGCAAGTCAGACCATCATGAACTATTTAAATTGGCAT3680.12498471654281405No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCAAAAGTGAGATAATCA3580.12158839272371584No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3530.11989023081416675No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT3500.11887133366843727No Hit
GATTTCACTCTCACCATCAACAATCTGCAACCTGAAGATTTTGCAGTTTA3420.1161542746131587No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT3340.11343721555788017No Hit
CAATAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA3280.11139942126642123No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGGCAT3250.11038052412069176No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCAAAAGTG3210.10902199459305248No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3190.10834272982923285No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT3190.10834272982923285No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG3170.1076634650654132No Hit
AGTCAGACCATCATGAACTATTTAAATTGGCATCAGCAGAAACCAGGGAA3110.10562567077395428No Hit
GTGCCAGATGTGGCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATTA3030.10290861171867571No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3020.10256897933676588No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2990.10155008219103641No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2950.10019155266339713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)