FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631248
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT56520.8953691734468863No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT54860.86907206042633No Hit
CTATAATATTGGTGACAATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC29280.4638430537601703No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28650.4538628241198388No Hit
CTCTTATACACCTCCGACAACAGGAACTACTTGGCTTGGTTGCAGCAGAA28140.44578359060147515No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25740.4077636681621169No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18740.2968722277139888No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17150.27168402909791395No Hit
CTTATACACCTCCGACAACAGGAACTACTTGGCTTGGTTGCAGCAGAAAC16980.26899095125845945No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16970.2688325349149621No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16840.26677312244949686No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16160.2560008110916787No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14190.2247927914227055No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14100.2233670443312296No Hit
GTATAAGAGACTCCGGCTGGACTTGCAGTTGATGGTGGCCCGCTCGCCCAGAGACACAGCCAGAGAGTCTGGAGA14040.2224165462702456No Hit
CCTTAGGCGGCTGTCCTGGTTTCTGCTGCAACCAAGCCAAGTAGTTCCTG13790.21845613768281244No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13420.21259473297341142No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13120.2078422426684916No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12820.20308975236357185No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11750.18613920360935796No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11260.178376802777989No Hit
GTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATA10990.1740995615035612No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10920.1729906470990799No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10390.16459458089372164No Hit
GTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGACAACAGGAACTA10330.16364408283273768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10030.15889159252781793No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9950.1576242617798393No Hit
AACCAGGACAGCCGCCTAAGGTGCTCATTTATTGGGCATCCACCCGGGAA9710.15382226953590347No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9560.1514460243834436No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9170.14526778698704787No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT8600.13623805540770031No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8470.13417864294223508No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8370.1325944795072618No Hit
CTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGC8330.13196081413327249No Hit
CATTTATTGGGCATCCACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA8280.13116873241578586No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC8190.12974298532430995No Hit
CTCTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGA8080.12800040554583936No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7920.12546574404988214No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTATTGTCACCAATATTATAGTGCT7920.12546574404988214No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7830.1240399969584062No Hit
ATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA7790.12340633158441691No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7550.11960433934048108No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7500.11881225762299444No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7420.11754492687501585No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7410.11738651053151852No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7380.11691126150102653No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7290.11548551440955061No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT7200.11405976731807467No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7160.11342610194408538No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7130.11295085291359339No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7080.11215877119610676No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7070.11200035485260944No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7040.11152510582211744No Hit
GTCAGGGACCCCGGATTCCCGGGTGGATGCCCAATAAATGAGCACCTTAGGCGGCTGTCCTGGTTTCTGCTGCAA6940.1099409423871442No Hit
GGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGACA6940.1099409423871442No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6930.10978252604364688No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6910.10946569335665221No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6880.10899044432616024No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6850.10851519529566826No Hit
CGCCTAAGGTGCTCATTTATTGGGCATCCACCCGGGAATCCGGGGTCCCT6770.10724786454768966No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC6700.10613895014320839No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6670.10566370111271639No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6560.1039211213342458No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC6370.10091121080779662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG501.8189894E-1161.2491234
CGCGTAT309.296147E-657.31571639
AAAGGGG8100.053.9162668
TCGTATG7700.052.3817440
CCGTCTT7800.052.27288447
CGTATGC7950.052.23106841
ACCGATC9000.051.6027427
TATCTCG8000.051.22210736
TATCGCG601.07320375E-1051.22210736
GTATCGC601.09139364E-1051.1313635
CAGTATC8700.050.7596933
ATGCCGT8200.050.61803444
AGACCGA9200.050.41680525
CCACTAG200.00821105750.3328741
TCGAACA200.00823165950.3009762
CACGAGA9300.050.16440221
TGCTTGA7450.050.1370155
CTTGAAA7800.050.1203257
ATCTCGT8000.050.0546237
CCGATCA9300.049.98184628
Produced by FastQC (version 0.11.5)