FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846654
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGTAGTGATTTCTACTGGGGCTTCGTCCGGCA28370.33508375322150485No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26670.3150047126689297No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23030.2720119434857687No Hit
GAGTATTAGTAACTGGGTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT21900.2586652871184687No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGACCTCAGGCTCATAGAGAACTGGTTCTTAGACGGGTCTGCTGA20930.2472084228031758No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19080.22535770220184398No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGTAGTGATTTCTACTGGGGCT18280.2159087419418086No Hit
GTATGGTACCTTTTCTCCGTACGCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18040.21307405386379796No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17820.21047558979228823No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16700.1972470454282387No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16280.19228634129172012No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16240.19181389327871834No Hit
GTTTATTACTGTGCGAGATCGGTAGACACAACGGTGGGTCACTTTGACTC15850.1872075251519511No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15770.18626262912594754No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15560.18378227705768826No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA15290.18059325296992634No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14100.1665379245831237No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14100.1665379245831237No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13730.16216778046285732No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13240.15638029230358566No Hit
GTCATGGACCTCCTGTGCAAGAGCATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC13230.1562621803003352No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13130.15508106026783078No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13130.15508106026783078No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13090.154608612254829No Hit
GTGCAAGAGCATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC13020.1537818282320759No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12970.1531912682158237No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12940.15283693220607236No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12640.14929357210855912No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12580.14858490008905645No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12580.14858490008905645No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAACTGGGTGGCCTGGTATCAGCAGAAA12190.1439785319622892No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12150.14350608394928743No Hit
GTACCATACTGTTGGCAGTGATAAGTACCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGACTGAATTCTGTC11950.14114384388427859No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11710.13830915580626799No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11670.1378367077932662No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11580.1367736997640122No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11510.13594691574125914No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT11420.13488390771200515No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11370.13429334769575293No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11350.13405712368925204No Hit
GTTCATCATAGTGGGACCACTTACTACAACCCGTCCTTCACGAGTCGGGT11240.1327578916534972No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11230.13263977965024673No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11130.1314586596177423No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11000.12992320357548656No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10940.12921453155598392No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10830.12791529952022904No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10720.12661606748447418No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10630.1255530594552202No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10580.12496249943896799No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10480.12378137940646355No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGCG10220.12071046732195206No Hit
GGAGTACATTGCGACTGTTCATCATAGTGGGACCACTTACTACAACCCGT9930.11728521922768924No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9910.11704899522118833No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGATTCCCTGG9900.1169308832179379No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9800.11574976318543348No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9770.11539542717568214No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9660.1140961951399273No Hit
GGCCAAAAGCGTACGGAGAAAAGGTACCATACTGTTGGCAGTGATAAGTACCAAAATCATCAGGCTGCAGGCTG9440.11149773106841755No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9390.11090717105216535No Hit
ACTTACTACAACCCGTCCTTCACGAGTCGGGTCACCATGTCAGCAGACCCGTCTAAGAACCAGTTCTCTATGAGC9380.1107890590489149No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9330.1101984990326627No Hit
GTCTCCTTCCGCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAA9190.10854493098715651No Hit
GAATTCAGTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGGTACTTA9150.10807248297415473No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9120.10771814696440342No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9050.10689136294165032No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8930.105474018902645No Hit
GTATTAGTAACTGGGTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT8930.105474018902645No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8890.10500157088964322No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8760.10346611484738748No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8750.10334800284413703No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8740.1032298908408866No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCGCC8590.10145821079212997No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCACGACTGGTGAAGCCTTCGGAGACCCTGCCCCTCACCTGCA8540.10086765077587775No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGATTCCCTGG8540.10086765077587775No Hit
CTATGAGCCTGAGGTCTGTGACCGCCGCAGACACGGCCGTTTATTACTGT8520.10063142676937688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTT150.002046620771.4878462
CGTATGC2100.054.39196441
GCCGTCT2050.054.2936746
ATGCCGT2150.053.2706744
GTATGCC2150.053.1684542
CTTATAC2150.052.407911
TCGTATG2200.051.85125440
CTACATC2000.051.4326733
TCTACAT2200.051.40216432
CGCTAAA200.00763548651.26834526
CACGAGA2200.051.18959421
GGTACGC200.007691006651.174488
GTGGTAT1950.050.7790761
ATCTCCG2250.050.03726610
ATCTCGT2100.049.18787837
CGAGACC2300.048.97842823
TACACAT2300.048.94955
CGTAATT400.00984268248.07402470
ACGAGAC2350.047.9306722
TATACAC2300.047.463383
Produced by FastQC (version 0.11.5)