FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846654
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGTAGTGATTTCTACTGGGGCTTCGTCCGGCA27780.3281151450297288No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25970.3067368724413987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23980.28323258379456073No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGACCTCAGGCTCATAGAGAACTGGTTCTTAGACGGGTCTGCTGA22390.26445277527774036No Hit
GAGTATTAGTAACTGGGTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT22250.26279920723223416No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20230.23894058257564482No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGTAGTGATTTCTACTGGGGCT17040.20126285353875376No Hit
GTATGGTACCTTTTCTCCGTACGCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16760.19795571744774132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16390.19358557332747497No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15980.18874298119420685No Hit
GTTTATTACTGTGCGAGATCGGTAGACACAACGGTGGGTCACTTTGACTC15640.1847271730836918No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15520.18330982904468648No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15310.1808294769764272No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14600.17244352474564578No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14460.1707899567001396No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA14270.1685458286383812No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14210.16783715661887855No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13890.1640575725148644No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13820.16323078849211128No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13740.16228589246610775No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13220.15614406829708474No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13050.15413616424182724No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12780.1509471401540653No Hit
GTACCATACTGTTGGCAGTGATAAGTACCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGACTGAATTCTGTC12620.1490573481020582No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11760.13889971582252017No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11620.137246147777014No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11450.13523824372175647No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11240.1327578916534972No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11190.13216733163724498No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10910.1288601955462326No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10890.12862397153973168No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10880.12850585953648125No Hit
GTCATGGACCTCCTGTGCAAGAGCATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10840.1280334115234795No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10800.12756096351047772No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10740.12685229149097507No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10730.1267341794877246No Hit
GTTCATCATAGTGGGACCACTTACTACAACCCGTCCTTCACGAGTCGGGT10620.12543494745196976No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10450.12342704339671225No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10380.12260025937395914No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAACTGGGTGGCCTGGTATCAGCAGAAA10380.12260025937395914No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10340.12212781136095736No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGATTCCCTGG10280.12141913934145471No Hit
GTGCAAGAGCATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC10230.1208285793252025No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10150.11988368329919896No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10120.11952934728944764No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10050.11870256326669455No Hit
ACTTACTACAACCCGTCCTTCACGAGTCGGGTCACCATGTCAGCAGACCCGTCTAAGAACCAGTTCTCTATGAGC10020.11834822725694322No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGCG9870.11657654720818658No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9800.11574976318543348No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9640.1138599711334264No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9630.11374185913017595No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9550.11279696310417242No Hit
GGAGTACATTGCGACTGTTCATCATAGTGGGACCACTTACTACAACCCGT9550.11279696310417242No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9520.1124426270944211No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9380.1107890590489149No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9240.1091354910034087No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9220.10889926699690783No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9160.10819059497740517No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9080.10724569895140164No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9070.1071275869481512No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGATTCCCTGG9030.10665513893514941No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9000.1063008029253981No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8810.10405667486363969No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8770.10358422685063792No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8770.10358422685063792No Hit
GTATTAGTAACTGGGTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT8720.10299366683438572No Hit
GTAGTAAGTGGTCCCACTATGATGAACAGTCGCAATGTACTCCAGTCCCTTCCCTGGGGGCTGCCGGACGAAGCC8650.10216688281163261No Hit
ATTAGTAACTGGGTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA8620.10181254680188129No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8590.10145821079212997No Hit
CTATGAGCCTGAGGTCTGTGACCGCCGCAGACACGGCCGTTTATTACTGT8580.1013400987888795No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8470.10004086675312465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)