FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177739
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38690.32851081606366095No Hit
GTAATACACGGCACTGTCGTCAGCCTTTAGGCTGCTGATCTGCAGATATG36560.31042531494669023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34390.2920001800059266No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC30510.25905569909801746No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26110.22169597848080091No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23450.19911032919857458No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23140.19647816706417973No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22250.18892131448478822No Hit
GTGTATTACTGTGCGAGAGATCGGGGGCGAAACTTTGACTACTGGGGCCA22020.18696841999797917No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTACTTGGCCTGGTACC21980.18662878617418632No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATT21730.1845060747754808No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGG20870.17720394756393396No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20590.1748265107973838No Hit
GTTTTACTGTCAACAATATGGTAGCTCATTTTACACTTTTGGCCAGGGGA20480.1738925177819534No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20040.17015654572023173No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGAGGCCAGAGGCCCTGGT19920.16913764424885308No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19690.16718474976204406No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19350.16429786225980458No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19180.16285441850868487No Hit
CATCTATGGTGCATCCACCAGGGCCTCTGGCCTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19120.16234496777299554No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18890.1603920732861865No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18410.15631646740067198No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18150.15410884754601825No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG17810.1512219600437788No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17560.14909924864507332No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17100.14519345967145522No Hit
ACCATATTGTTGACAGTAAAACACTGCAAAATCTTCAGGCTCCAGTCTGC17070.14493873430361057No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17020.14451419202386948No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGAGGCCA16930.14375001592033548No Hit
GTGCAGCCACAGTTCGTTTCATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16850.14307074827274974No Hit
CTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTTTTACTGTCAAC16660.14145748760973356No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16530.1403536776824067No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16530.1403536776824067No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCAGCTTGGTCC16430.13950459312292451No Hit
GACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTT16330.13865550856344233No Hit
GTCAGAGTGTTAGCGGCAACTACTTGGCCTGGTACCAGCACAAACCTGGC15930.13525917032551354No Hit
CCATAGATGAGAAGCCTGGGAGGCTGGCCAGGTTTGTGCTGGTACCAGGCCAAGTAGTTGCCGCTAACACTCTGA15870.13474971958982424No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT15470.13135338135189545No Hit
GCACAAACCTGGCCAGCCTCCCAGGCTTCTCATCTATGGTGCATCCACCAGGGCCTCTGGCCTCCCAGACAGGTT15160.12872121921750065No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15120.12838158539370778No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14950.12693814164258804No Hit
CAGTAAAACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14930.1267683247306916No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14720.12498524715577901No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14620.1241361625962968No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14560.12362671186060749No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14300.12141909200595379No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13760.11683403538474994No Hit
GGTTTGTGCTGGTACCAGGCCAAGTAGTTGCCGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC13670.11606985928121595No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13560.11513586626578554No Hit
CTGCTACTCTGGCTCCCAGATACCAGCGGAGAAATTGTGTTGACGCAGTC13510.11471132398604444No Hit
CAGTAATACACGGCACTGTCGTCAGCCTTTAGGCTGCTGATCTGCAGATA13470.11437169016225157No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13460.11428678170630334No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13190.11199425339570143No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13160.11173952802785676No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13080.11106026038027102No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13080.11106026038027102No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12920.1097017250850995No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12660.1074941052304458No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12500.10613556993527427No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12450.10571102765553318No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCAGCGGAGAAATTGTGTTGACGCAGTCTC12230.10384304162467237No Hit
CTACTTGGCCTGGTACCAGCACAAACCTGGCCAGCCTCCCAGGCTTCTCATCTATGGTGCATCCACCAGGGCCT12100.10273923169734551No Hit
GTCTGGGAGGCCAGAGGCCCTGGTGGATGCACCATAGATGAGAAGCCTGGGAGGCTGGCCAGGTTTGTGCTGGTA12020.10205996404975975No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11910.10112597103432933No Hit
GTATTACTGTGCGAGAGATCGGGGGCGAAACTTTGACTACTGGGGCCAGG11910.10112597103432933No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11860.10070142875458823No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAACTGTGGCTGCACCA11850.10061652029864003No Hit
GTACCAGCACAAACCTGGCCAGCCTCCCAGGCTTCTCATCTATGGTGCATCCACCAGGGCCTCTGGCCTCCCAGA11840.1005316118426918No Hit
GGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCTCCATCAGCAGA11810.10027688647484714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA354.5001658E-449.2658269
TCGTATG2450.045.0633940
GGTATCA10200.045.0626561
ATCTCGT2350.040.9181437
GACTAGG2600.040.62079226
TACACAT2850.040.6006365
CCCACGA2900.039.90909219
ACGAGAC2750.039.61367822
AGACTAG2700.039.10634225
TAGGCAT2800.038.972529
ATGCCGT2900.038.2883444
TATACAC3200.038.2837453
ATCTCCG2950.038.072310
TATGCCG3100.037.9942643
ACTAGGC2800.037.7257227
CGAGACT2950.036.93113723
CACGAGA3150.036.74326721
GAGACTA3000.036.32333424
GCCGTCT3200.034.82998746
CTTATAC3350.034.555511
Produced by FastQC (version 0.11.5)