FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177739
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38540.3272371892244377No Hit
GTAATACACGGCACTGTCGTCAGCCTTTAGGCTGCTGATCTGCAGATATG34420.29225490537377125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34290.2911510954464444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28740.24402690239518263No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC27060.2297622817958818No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24830.21082769611942884No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23200.19698761779986906No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATT22390.1901100328680633No Hit
GTGTATTACTGTGCGAGAGATCGGGGGCGAAACTTTGACTACTGGGGCCA21870.1856947931587559No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21840.18544006779091124No Hit
GTTTTACTGTCAACAATATGGTAGCTCATTTTACACTTTTGGCCAGGGGA20660.17542086998902134No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTACTTGGCCTGGTACC20610.17499632770928025No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20400.17321325013436764No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20280.172194348662989No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGG19040.1616657001254098No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGAGGCCAGAGGCCCTGGT18470.15682591813636126No Hit
CATCTATGGTGCATCCACCAGGGCCTCTGGCCTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18170.1542786644579147No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18090.15359939681032894No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17960.15249558688300208No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17620.14960869938076263No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17230.14629726959878206No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17160.14570291040714453No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16810.14273111444895686No Hit
ACCATATTGTTGACAGTAAAACACTGCAAAATCTTCAGGCTCCAGTCTGC16450.13967441003482098No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16160.1372120648123226No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGAGGCCA16050.1362780717968922No Hit
CTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTTTTACTGTCAAC15960.13551389569335823No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15940.13534407878146176No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG15870.13474971958982424No Hit
GTGCAGCCACAGTTCGTTTCATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15680.13313645892680806No Hit
GACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTT15590.1323722828232741No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15340.1302495714245686No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15270.12965521223293106No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15220.12923066995318996No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14900.12651359936284695No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCAGCTTGGTCC14720.12498524715577901No Hit
CCATAGATGAGAAGCCTGGGAGGCTGGCCAGGTTTGTGCTGGTACCAGGCCAAGTAGTTGCCGCTAACACTCTGA14710.12490033869983078No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14690.12473052178793434No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14650.12439088796414145No Hit
GTCAGAGTGTTAGCGGCAACTACTTGGCCTGGTACCAGCACAAACCTGGC14590.12388143722845216No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14370.12201345119759131No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14210.12065491590241981No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14120.11989073979888584No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13990.11878692987155898No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13950.11844729604776609No Hit
GGTTTGTGCTGGTACCAGGCCAAGTAGTTGCCGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC13930.11827747913586967No Hit
GCACAAACCTGGCCAGCCTCCCAGGCTTCTCATCTATGGTGCATCCACCAGGGCCTCTGGCCTCCCAGACAGGTT13810.11725857766449103No Hit
CAGTAAAACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13780.11700385229664638No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13280.1127584294992354No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13190.11199425339570143No Hit
CTGCTACTCTGGCTCCCAGATACCAGCGGAGAAATTGTGTTGACGCAGTC13000.11038099273268526No Hit
GTGTTTTACTGTCAACAATATGGTAGCTCATTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAA12760.10834318978992798No Hit
CAGTAATACACGGCACTGTCGTCAGCCTTTAGGCTGCTGATCTGCAGATA12320.10460721772820633No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCAGCGGAGAAATTGTGTTGACGCAGTCTC12060.10239959787355261No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12040.10222978096165619No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11970.10163542177001866No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11960.10155051331407043No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11780.10002216110700247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)