FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678183
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35540.5240473441534217No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21810.32159461384316623No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT17610.2596644268582374No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC15960.2353347105427296No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15930.23489235206426584No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC15900.23444999358580207No Hit
CCACTACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT15290.2254553711903719No Hit
GTAATAAACGGCCGTGTCGTCGACTTTCAGGCTGTTCATTTGCAGGTACA14740.2173454657518693No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG13950.20569669248565653No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13300.19611225878560803No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13130.19360556074097995No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTACACAGGGTCTCT13030.19213103247943403No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG12940.19080395704404268No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG12650.18652782508555948No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12240.18048225921322122No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT12210.18003990073475745No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12030.17738574986397476No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC11840.1745841461670375No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11360.16750641051161708No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11210.1652946181192982No Hit
ACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG11130.16411499551006145No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC11110.16382008985775226No Hit
GCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT11030.16264046724851552No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTCATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC10630.15674235420233182No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT10570.15585763724540425No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG10430.15379329767923997No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC10320.15217131659153946No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9770.14406141115303686No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9470.13963782636839908No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC9460.1394903735422445No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATG9290.13698367549761642No Hit
GTCGTATAGCCACTGCTATACCCGGTCCTCGCACAGTAATAAACGGCCGT9280.13683622267146184No Hit
GTTTATTACTGTGCGAGGACCGGGTATAGCAGTGGCTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGT9240.13624641136684346No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC9220.1359515057145343No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG9220.1359515057145343No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG8940.1318228265822057No Hit
CTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGTTGTGC8920.1315279209298965No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG8920.1315279209298965No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8900.13123301527758732No Hit
CCTGCACTCTGAGCGGTGGGCACAACAGCTACGCCATCGCATGGCATCAG8890.13108556245143274No Hit
GTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG8770.12931612853757762No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG8560.12621961918833116No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT8420.12415527962216688No Hit
CACCTGCACTCTGAGCGGTGGGCACAACAGCTACGCCATCGCATGGCATC8360.12327056266523931No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTACA8240.12150112875138422No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8160.12032150614214748No Hit
GCTATACCCGGTCCTCGCACAGTAATAAACGGCCGTGTCGTCGACTTTCA8140.12002660048983829No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC8030.11840461940213777No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA7840.1156030157052005No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7780.11471829874827295No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG7480.11029471396363519No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7430.10955744983286222No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7310.10778801591900711No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTA7280.10734565744054334No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7280.10734565744054334No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7280.10734565744054334No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCATTCATGTCTTCGGAACTGGGACCAAGGT7270.10719820461438875No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC7230.10660839330977037No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTACACAGGGT7140.10528131787437905No Hit
CTGCTATACCCGGTCCTCGCACAGTAATAAACGGCCGTGTCGTCGACTTT7110.10483895939591525No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG6890.10159499722051422No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC6870.10130009156820503No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC6840.10085773308974126No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC6800.10026792178512291No Hit
GGGTATAGCAGTGGCTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGC6790.1001204689589683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA252.7246162E-454.53561811
CGTACAT200.00771628751.130912
ACTGACG200.007718536551.127148
GGTATCA7300.049.5404241
AATGGTA1409.094947E-1248.93063470
GCGATTT659.690381E-442.15562470
GTATCAA19650.040.628431
TATCAAC22950.034.458592
TGGTATC1600.034.0872732
AACGCAG24300.032.822366
ATCAACG24200.032.673893
TCAACGC24350.032.4726144
GTGGTAT1700.032.1105541
CAACGCA24850.031.8215855
CGCAGAG25750.030.9741128
AGAGTAC26150.030.50031911
CAGAGTA26350.030.26881810
GCAGAGT26500.030.2261059
TTCCGTG605.7329616E-428.47535530
CTTGTCA605.815271E-428.4060612
Produced by FastQC (version 0.11.5)