FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139695
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT49100.430817016833451No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGATCCA47540.4171291442008607No Hit
GTTAATATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT46080.40431869930112885No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37640.33026379864788385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33610.29490346101369225No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30390.2666502880156533No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28190.24734687789276957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23860.20935425706000288No Hit
GTATATTACTGTGCGAAAGATCCTGGCGGGGGATGGGGGGTGCCTTCCACGGGTGACTACTGGGGCCAGGGAACC23750.2083890865538587No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23250.20400194788956694No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCGCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA21430.18803276315154493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20970.18399659558039652No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20830.18276819675439482No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20100.17636297430452882No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGCGATACCAGTGGCCCTGGT19920.17478360438538382No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATATTAACTGG19840.17408166219909713No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19740.17320423446623875No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT19720.1730287489196671No Hit
CCATAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTCTGCTGGTACCAGGC18790.16486867100408442No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGACTCCCAGGCT18740.16442995713765526No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18710.16416672881779776No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18190.1596041046069343No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18030.15820022023436095No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17740.15565567980907172No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACCACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG17410.15276016829063915No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTA17340.15214596887763832No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17010.14925045735920575No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16840.14775883021334654No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16840.14775883021334654No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16460.1444246048284848No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15820.13880906733819137No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15500.13600129859304463No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15460.1356503274999013No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGCGATACCA15250.13380772926089873No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15210.1334567581677554No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14850.13029801832946533No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG14630.12836767731717696No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14590.1280167062240336No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCGCA14460.12687605017131776No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14420.12652507907817442No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14410.1264373363048886No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13980.12266439705359768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13950.12240116873374017No Hit
GTTTATTACTGTCAGCAGTATATTAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13650.11976888553516513No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA13550.11889145780230677No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13480.1182772583893059No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGACTCCCAGGCTCCTCATCTATGGTG13200.11582046073730252No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13180.11564497519073084No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12590.11046815156686657No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC12560.11020492324700906No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA12090.10608101290257481No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11930.10467712853000144No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGATC11770.10327324415742808No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11550.1013429031451397No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC11510.10099193205199636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTA408.116465E-569.6633570
CGTTTAA350.00417631359.7114470
TCTGGAG14100.056.81762370
GTATCAA19600.039.5308881
CACCGTT902.1664164E-937.72436567
TCGGTTT1001.7584654E-434.83167670
CGTAGGT400.002739220934.12504634
CTTGTTA601.5433812E-533.8046072
GCCATAG502.099028E-433.801643
ATTGTCT14300.032.66674864
GAATTGT14300.032.4210262
AATTGTC14450.031.94796263
TATCAAC24150.031.9149112
GGAATTG14650.031.79354561
ATCAACG24550.031.6675263
AACGCAG24500.031.5955756
CGTGTTC14750.031.55372652
TCAACGC24750.031.2736824
ACCGTTT1151.3860699E-831.1928868
GCGTGTT14950.031.06053251
Produced by FastQC (version 0.11.5)