FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701878
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAATACTTACCCTCTTTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT24450.34835113794705064No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22980.3274073271993138No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22910.32641000287799304No Hit
GTATTAAGCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTGTGTC22690.3232755550109848No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15420.21969630049666752No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15310.21812907656316338No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15130.21556452830833847No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14160.20174446271289315No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13540.19291101872405175No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12960.1846474743473937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12610.17966085274078972No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12490.17795115390423977No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12390.1765264048737815No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12350.17595650526159817No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT11900.16954513462453588No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11130.15857456709000708No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11110.15828961728391544No Hit
GATCTATGCTGCTTCCACTTTGCTAGGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACACAATTCA10470.14917122348898243No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10220.1456093509128367No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10210.14546687600979089No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10040.14304480265801178No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9970.14204747833669099No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTCTTTAGCCTGGTATCAGCAAAAC9940.1416200536275535No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT9900.1410501540153702No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9850.14033777950014106No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9530.13577858260267453No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9480.1350662080874454No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9430.13435383357221625No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9040.12879731235342892No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8790.12523543977728321No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8410.11982139346154175No Hit
GGGCATTAGCAGTTCTTTAGCCTGGTATCAGCAAAACCCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCTTC8190.11668694559453352No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8150.11611704598235022No Hit
GTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTGTGTCCCAGATCCACTGCCGCTGAACCGTGATGGGAC8140.11597457107930438No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8000.11397992243666279No Hit
ACTTATTACTGTCAACAGCTTAATACTTACCCTCTTTTCGGCCAAGGGAC7960.11341002282447947No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT7750.11041804986051708No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7500.10685617728437136No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7450.10614380276914222No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7360.10486152864172976No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC7260.10343677961127148No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7250.10329430470822565No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7240.10315182980517983No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7150.10186955567776737No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAGGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAA7130.10158460587167571No Hit
CAATAATACACGGCCGTGTCTTCAGATGTCAGGCTGCTCAGCTCCATGTA7050.10044480664730907No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7040.10030233174426326No Hit
GCCTGAAGACTTTGCAACTTATTACTGTCAACAGCTTAATACTTACCCTC7020.1000173819381716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA2200.044.780482
CTTATAC2250.043.8040771
ACGAGAC2350.043.40484622
TATCTCG2100.042.54783236
TCGTATG2200.042.45741340
ATCTCGT2150.041.60032737
CGTATGC2250.041.56215741
CACGAGA2350.040.50830521
CGAGACG2450.040.24831423
TATGCCG2350.039.90659743
ACGGACT2500.039.50814427
GACGGAC2500.039.49122226
TATACAC2550.038.6341363
TATTTAC400.002097104236.0326862
TACACAT2800.035.184665
CCTATCT2550.034.96631634
CTCCTAT2650.034.8682232
ACTCCTA2650.034.81824531
GTATCAA19250.034.42731
CTATCTC2600.034.3136335
Produced by FastQC (version 0.11.5)