FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099598
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42500.3865048863311865No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38800.3528562256388244No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30540.2777378642012808No Hit
CTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGG28760.2615501301384688No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27070.2461808770114169No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25390.23090256621056057No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23800.21644273634546443No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23510.21380540888579277No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22880.20807604233547167No Hit
GTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTT22730.2067119074425381No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21860.1987999250635232No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20500.18643176870092526No Hit
CAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTGCCAGACCCACTGCCA20490.18634082637472968No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20430.18579517241755625No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20250.18415821054603593No Hit
GTCTCCAGGCATCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCTGGGCCAGTCAGGGTGTTAGCAG19790.17997486354103953No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAATCTA18940.1722447658144158No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT18330.16669728391648583No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18000.1636961871520319No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17230.1566936280349728No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17180.1562389164039949No Hit
ACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACGGACTTCACTCTCA16780.152601223356172No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16730.1521465117251941No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16240.14769033774161103No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCC15980.14532583726052611No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15590.14177908653889876No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15490.14086966327694303No Hit
GGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14780.13441275811705733No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14600.132775796245537No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14550.13232108461455913No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14530.132139199962168No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14510.13195731530977686No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14480.13168448833119012No Hit
GGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAGCCTGGCCAGG14180.12895621854532294No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13980.12713737202141145No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13750.12504569851891328No Hit
ATCTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGAC13710.12468192921413097No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13580.12349967897358853No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13480.12259025571163279No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13460.12240837105924164No Hit
GTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAGCCTGGC13430.12213554408065494No Hit
CTCCTGCTGGGCCAGTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACC13360.12149894779728591No Hit
ATGTATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGGAGTTGCTGCTAACAC13260.12058952453533019No Hit
GAGTGAAGTCCGTGCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA12660.1151329849635958No Hit
CTTCCATACTGCTGACAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTG12620.11476921565881348No Hit
GTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTGCCAGACCCACTGCCA12330.11213188819914188No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12290.11176811889435957No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12120.11022209934903482No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTCTGACGCAGTCTC11960.10876702212990566No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11930.10849419515131893No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11810.10740288723697206No Hit
GCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTT11690.10631157932262517No Hit
GTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCTGGGCCAGTCAGG11570.1052202714082783No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11530.10485650210349601No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11510.10467461745110485No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11450.10412896349393141No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11200.10185540533904208No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11170.10158257836045535No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC11130.10121880905567307No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT11100.10094598207708635No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11070.10067315509849964No Hit
CCTCCACCCTGGTCCCTCCGCCGAAAGTGGAAGAGCTTCCATACTGCTGA11040.10040032811991291No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCCCTCCGCCGAAAGTG11030.10030938579371734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)