FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900346
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28610.3177667252367423No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28150.3126575783087835No Hit
CTATTACTGTCAGCAGTATAGTCACTCACCTCGGACGTTCGGCCAGGGGA25000.2776710286934134No Hit
GTAATAAACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA24340.2703405135359073No Hit
CTCCTGCAGAGCCAGTCAGAGTCTTTACAACGGCCACTTAGCCTGGTACC21910.24335088954690753No Hit
GTATAGTCACTCACCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT21310.23668678485826558No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19740.21924904425631922No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA19710.21891583902188716No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT19530.21691660761529458No Hit
GTCTATTACTGTCAGCAGTATAGTCACTCACCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAA19490.21647233396938512No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19440.21591699191199826No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19090.2120295975102905No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGAGCCAGTCAGAGTCTTTACAA17800.19770177242971035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17750.19714643037232354No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCCGACCCACTGCCA16980.1885941626885664No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16580.18415142622947178No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15750.17493274807685047No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCCGACCCACTGCCA15740.1748216796653731No Hit
GTTTATTACTGTGCGAGAGGTGATGGTGGCGACAACTGGTTGATTGACTA15450.1716006957325295No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15250.16937932750298218No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15000.16660261721604805No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14460.16060492299627033No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGAGCCAGTCAGA14340.15927210205854192No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13570.1507198343747848No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13260.14727671361898648No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC12370.13739162499750096No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12150.13494811994499895No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGAGCCAGTCAGAGTCTTTACAACGGCCACTTAGCCTGGTACCAGCAGAAA12150.13494811994499895No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12040.1337263674187479No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12010.13339316218431582No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11770.13072752030885904No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11430.1269511943186286No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTATAGTCACTCA11160.12395234720873975No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC11040.12261952627101137No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11000.12217525262510191No Hit
GTGACTATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGT10840.12039815804146406No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10730.11917640551521304No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA10710.11895426869225831No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10690.11873213186930358No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10610.11784358457748464No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10230.11362298494134476No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10030.11140161671179746No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9830.10918024848225016No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC9730.1080695643674765No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9680.10751422231008968No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9620.10684781184122549No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9550.10607033296088392No Hit
CCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9520.10573712772645183No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9510.10562605931497447No Hit
GAGTGAAGTCCGTCCCCGACCCACTGCCACTGAACCTGTCTGGGATGCCA9320.10351575949690453No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCGTCATCCATCAAGCAACCAACAGGGCCACTGGCATC9290.10318255426247243No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9210.1022940069706535No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9120.10129439126735722No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9050.10051691238701566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)