FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900346
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38570.4283908630681983No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28890.32087664075810857No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25000.2776710286934134No Hit
CTATTACTGTCAGCAGTATAGTCACTCACCTCGGACGTTCGGCCAGGGGA22380.2485711048863437No Hit
CTCCTGCAGAGCCAGTCAGAGTCTTTACAACGGCCACTTAGCCTGGTACC21290.23646464803531086No Hit
GTATAGTCACTCACCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT20860.23168870634178415No Hit
GTAATAAACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA20670.2295784065237142No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20150.22380284912689122No Hit
GTCTATTACTGTCAGCAGTATAGTCACTCACCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAA18960.21058570816108474No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18870.20958609245778845No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA18700.20769792946267324No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17450.19381437802800258No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17130.19026018886072688No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGAGCCAGTCAGAGTCTTTACAA16520.18348501576060758No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15770.17515488489980519No Hit
GTTTATTACTGTGCGAGAGGTGATGGTGGCGACAACTGGTTGATTGACTA15590.1731556534932126No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCCGACCCACTGCCA15140.16815757497673117No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGAGCCAGTCAGA15100.1677133013308217No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCCGACCCACTGCCA15060.16726902768491225No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14640.16260415440286288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13680.15194158690103585No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13640.15149731325512636No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13400.1488316713796696No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13380.14860953455671486No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGAGCCAGTCAGAGTCTTTACAACGGCCACTTAGCCTGGTACCAGCAGAAA13120.14572175585830335No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13050.1449442769779618No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC12280.13639200929420467No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11770.13072752030885904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11720.1301721782514722No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11400.12661798908419652No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11240.12484089450055867No Hit
GTGACTATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGT10770.1196206791611225No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTATAGTCACTCA10610.11784358457748464No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA10500.11662183205123364No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC10440.11595542158236943No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10440.11595542158236943No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10390.11540007952498262No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10340.1148447374675958No Hit
CTCCTGTGGAGCCTCTGCATTCACCTTCAGTGCTCATAGCATGAACTGGGTCCGCCAGGCTCCAGGGCGGGGG9580.10640353819531602No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9520.10573712772645183No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCGTCATCCATCAAGCAACCAACAGGGCCACTGGCATC9510.10562605931497447No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9480.10529285408054237No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9350.10384896473133662No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC9130.10140545967883459No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9090.10096118603292513No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9080.10085011762144774No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9060.10062798079849301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTA200.006339802453.73689356
GGTATCA10500.039.783481
TCTGGAG7450.038.05953270
GTGGTAT3650.036.9179461
GTATCAA24600.036.0156331
GTCACCG7000.035.68404870
GTCGGTT500.002307918635.3362869
TTTCGAT1358.6737964E-735.0053770
TGGTATC4200.032.054942
TCGGTTT850.003886189331.7695870
TATCAAC29300.029.7520182
AACGCAG29650.029.4024456
ATCAACG29750.029.2987333
CAACGCA29850.029.0910665
TCAACGC29850.029.0878354
GTGAGTT5850.028.42588251
TCGTCTC5750.028.1359220
CGCAGAG31000.028.1220158
AGAGTAC31300.027.63740211
GCAGAGT31600.027.375029
Produced by FastQC (version 0.11.5)