FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880458
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49610.563456746375182No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45880.5210924314390919No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGATCCTGTCAGGGACACTG34480.39161436434219465No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33840.3843454202244741No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31320.3557239527609494No Hit
GATTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACT31310.35561037550911007No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG30530.3467513498656381No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29990.3406181782663114No Hit
GTCTACAAACTCCGACTTTTGGTCAGGGGACCAAGCTAGAGATCAAACGA29760.3380059014740056No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA28680.3257395582753521No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG28420.3227865497275282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28410.3226729724756888No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27160.30847581599576585No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26580.3018883353890816No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTC26210.2976859770710244No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26020.2955280092860761No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25810.29314288699744906No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25600.290757764708822No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT25090.28496532486501347No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24740.28099012105063503No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22620.25691174366068564No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21950.24930206778744698No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21010.23862580611454495No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA20320.23078897573762747No Hit
GTCCCTGACAGGATCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAG20180.22919889421187611No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT20000.22715450367876716No Hit
CTCCACAACTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCT19380.2201127140647254No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19090.2168189737613833No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGACCAAAAGTC18690.21227588368780792No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18480.2098907613991809No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18390.20886856613262644No Hit
GCCCTCCAGTGTCCCTGACAGGATCAGTGGCAGTGGATCAGGCACAGATT18240.20716490735503568No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18230.20705133010319626No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC17430.1979651499560456No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC17280.19626149117845487No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAAT17070.1938763688898278No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17020.19330848263063088No Hit
GTCAGGGACACTGGAGGGCCGATTGGAACCCAAATAGATCAGGAGTTGTGGAGTCTGCCCTGGCTTCTGCAGGTA16620.18876539255705552No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16610.18865181530521613No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC16570.1881975062978586No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16520.1876296200386617No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC16040.18217791195037128No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15960.18126929393565622No Hit
GTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTT15790.1793384806543867No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15460.17559043134368704No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT15290.1736596180624175No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA15210.17275100004770244No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14950.16979799149987848No Hit
GCATAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC14850.16866221898148465No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14790.16798075547044833No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCGACTTTTGGTCAGGGGAC14690.1668449829520545No Hit
GTTGTGGAGTCTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTAACCATTACTATGCAGGAGGCTCTGA14410.16366481990055176No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14260.161961161122961No Hit
AGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTTCCAATCGGCCC14200.1612796976119247No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCGACTTTTGGTCAGGGGACCAAGCTAGAGATCAAACGAACTGTGG14160.1608253886045672No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13770.1563958757828312No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13770.1563958757828312No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13670.15526010326443737No Hit
CTGGAGGGCCGATTGGAACCCAAATAGATCAGGAGTTGTGGAGTCTGCCC13550.15389717624236476No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13220.15014912693166513No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13120.14901335441327127No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13120.14901335441327127No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13080.14855904540591375No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13010.14776400464303804No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12530.14231229655474764No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12500.1419715647992295No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG12310.1398135970142812No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12300.1397000197624418No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAA12250.1391321335032449No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12250.1391321335032449No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12140.1378827837330117No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12060.1369741657182966No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT11880.13492977518518773No Hit
AGTCAGAGCCTCCTGCATAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTCCACAA11740.13333969365943635No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11670.13254465289656064No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11630.13209034388920313No Hit
CTCCTGCATAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTCCACAACTCCTGAT11600.13174961213368497No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11590.13163603488184558No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11580.1315224576300062No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11580.1315224576300062No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11110.12618432679355518No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11030.12527570877884012No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11010.12504855427516134No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11000.12493497702332196No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10970.1245942452678038No Hit
CTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCTGACAGGAT10880.12357205000124935No Hit
GTCTGGGACTGAGGTGAAGACGCCAGGGTCCTCGGTGAAGGTCTCCTGTAGGCCTTCTGGGGGCATTATCAGTAA10870.12345847274940996No Hit
TAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGA10780.12243627748285552No Hit
CCACAACTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCTGA10760.12220912297917674No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10730.12186839122365858No Hit
CCAGTGTCCCTGACAGGATCAGTGGCAGTGGATCAGGCACAGATTTTACA10680.12130050496446168No Hit
GCAGAAGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCTGACAGGAT10670.1211869277126223No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC10660.12107335046078291No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10620.12061904145342538No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10620.12061904145342538No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10570.12005115519422846No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10540.11971042343871031No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG10520.11948326893503154No Hit
GTACCTGCAGAAGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCTGA10410.11823391916479832No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG10390.11800676466111955No Hit
GAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGC10370.11777961015744078No Hit
GGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTTCCAAT10350.11755245565376203No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTAACCATTACTATGC10350.11755245565376203No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACTCCGACTTTTGGTCAGGGGACCAAG10300.1169845693945651No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10280.11675741489088633No Hit
TTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC10280.11675741489088633No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10260.11653026038720757No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA10150.11528091061697435No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9800.11130570680259592No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9650.10960204802500516No Hit
GATCAGGAGTTGTGGAGTCTGCCCTGGCTTCTGCAGGTACCAATCCAAAT9560.10857985275845071No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT9500.10789838924741442No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9410.10687619398085996No Hit
GGCACAGATTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTA9360.10630830772166305No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9260.10517253520326922No Hit
CAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTA9060.10290099016648153No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8890.10097017688521202No Hit
CTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTTCCAATCGGCCCTC8850.10051586787785448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)