FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880458
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50060.5685677227079543No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36910.4192136365391648No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGATCCTGTCAGGGACACTG33610.38173314343216824No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31380.35640541627198574No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30060.3414132190291871No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA28980.32914687583053365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28650.325398826519834No Hit
GATTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACT27920.317107687135559No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG27590.3133596378248593No Hit
GTCTACAAACTCCGACTTTTGGTCAGGGGACCAAGCTAGAGATCAAACGA27320.31029305202519597No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27150.30836223874392643No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25910.29427865951584287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25860.29371077325664596No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24750.2811036983024744No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTC24630.2797407712804018No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24600.2794000395248836No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23820.2705410138814117No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23360.26531646029680006No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23350.2652028830449607No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22140.25146003557239527No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20870.23703572458879354No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20690.23499133405568465No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20600.2339691387891302No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20550.2334012525299333No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG20070.2279495444416429No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19750.2243150723827826No Hit
CTCCACAACTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCT19120.21715970551690142No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT18620.21148084292493224No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18590.2111401111694141No Hit
GTCCCTGACAGGATCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAG18470.20977718414734148No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18280.20761921636239322No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA18280.20761921636239322No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAAT17400.19762441820052745No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA17330.19682937743765178No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGACCAAAAGTC17050.19364921438614904No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC16870.19160482385304012No Hit
GCCCTCCAGTGTCCCTGACAGGATCAGTGGCAGTGGATCAGGCACAGATT16670.18933327881625245No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16600.18853823805337677No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC16500.18740246553498294No Hit
GTCAGGGACACTGGAGGGCCGATTGGAACCCAAATAGATCAGGAGTTGTGGAGTCTGCCCTGGCTTCTGCAGGTA15920.18081498492829867No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCGACTTTTGGTCAGGGGAC15530.17638547210656272No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15370.1745682360771326No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15150.17206953653666612No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15040.17082018676643293No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14910.16934368249252094No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14890.1691165279888422No Hit
GTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTT14830.16843506447780587No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14550.1652549014263031No Hit
GCATAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC14510.1648005924189456No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC14420.16377839715239115No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14230.16162042936744286No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14230.16162042936744286No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT14200.1612796976119247No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC13960.1585538435677795No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13830.1570773392938675No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCGACTTTTGGTCAGGGGACCAAGCTAGAGATCAAACGAACTGTGG13740.15605514402731305No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13380.15196636296109525No Hit
CTGGAGGGCCGATTGGAACCCAAATAGATCAGGAGTTGTGGAGTCTGCCC13180.14969481792430758No Hit
GTTGTGGAGTCTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTAACCATTACTATGCAGGAGGCTCTGA13140.14924050891695004No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13120.14901335441327127No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12950.14708254113200175No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12830.14571961410992915No Hit
AGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTTCCAATCGGCCC12730.1445838415915353No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12450.14140367854003258No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12380.1406086377771569No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12170.13822351548852982No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12140.1378827837330117No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12140.1378827837330117No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG12070.13708774297013598No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11970.13595197045174215No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11880.13492977518518773No Hit
CTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCTGACAGGAT11850.13458904342966957No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11750.13345327091127573No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11750.13345327091127573No Hit
AGTCAGAGCCTCCTGCATAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTCCACAA11750.13345327091127573No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11570.1314088803781668No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11400.1294780670968973No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11290.12822871732666408No Hit
GTCTGGGACTGAGGTGAAGACGCCAGGGTCCTCGGTGAAGGTCTCCTGTAGGCCTTCTGGGGGCATTATCAGTAA11230.12754725381562776No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11220.1274336765637884No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAA11200.12720652206010963No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG10920.1240263590086069No Hit
CTCCTGCATAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTCCACAACTCCTGAT10820.12289058649021306No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10780.12243627748285552No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10750.12209554572733736No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10680.12130050496446168No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACTCCGACTTTTGGTCAGGGGACCAAG10670.1211869277126223No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10660.12107335046078291No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10610.12050546420158599No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC10400.11812034191295893No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10360.11766603290560139No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10350.11755245565376203No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10290.11687099214272571No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT10280.11675741489088633No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10280.11675741489088633No Hit
CCACAACTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCTGA10070.11437229260225928No Hit
TAGTAATGGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGA9910.11255505657282913No Hit
GAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGC9770.11096497504707777No Hit
GGTTACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTTCCAAT9700.11016993428420209No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9680.10994277978052332No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9650.10960204802500516No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9340.10608115321798428No Hit
CCAGTGTCCCTGACAGGATCAGTGGCAGTGGATCAGGCACAGATTTTACA9290.10551326695878735No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9220.10471822619591167No Hit
GCAGAAGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCTGACAGGAT9210.10460464894407229No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTAACCATTACTATGC9210.10460464894407229No Hit
GTACCTGCAGAAGCCAGGGCAGACTCCACAACTCCTGATCTATTTGGGTTCCAATCGGCCCTCCAGTGTCCCTGA8970.10187879489992709No Hit
GAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATG8950.10165164039624831No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8860.10062944512969386No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA8810.10006155887049693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1550.052.7556341
CGTTGTA200.007686232751.18267426
TAGGTGT200.007729576451.1099318
GGTATCA6700.046.276011
TGGTATC1900.042.9983832
GTATCAA17250.040.68811
CCTTATA451.0778683E-437.857051
CTTATAG451.08365384E-437.822652
GACAGGT1400.034.0713461
TATCAAC20750.033.630262
ATCAACG21200.032.589723
TCAACGC21300.032.276934
TGTTAGA11350.032.08672770
CAACGCA21650.031.7605425
AACGCAG21600.031.6782676
GGGACTA550.004217849631.24483769
GAATGGA850.004272666331.1601670
CTCGTAT1151.2223609E-930.94308760
TTATAGA553.5263255E-430.9405333
ACGCAGA22650.030.209747
Produced by FastQC (version 0.11.5)