FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857672
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATACTTTCCCCCTCACTTTCGGCGGGGGGACCAAGGTGGACGTCAGACGAACTGTGGCTGCACCAT46680.5442640076859219No Hit
GTATGATACTGTTGGCAGTAATAAGTCGCAAAATCTTCGGGCTGGAGGCTGGTGATGGTGAGAGTGAAGTCCGTC44210.515465119532875No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31990.3729864097230643No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30160.35164958165825627No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21590.2517279332891828No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21440.2499790129560018No Hit
GGTCTGGAGTGGGTGGCAGTCATCTCCTTTGATGGATCCAAGACGTTCTA19430.22654348049137665No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19380.225960507046983No Hit
GGACATAGGTGATTATTTAGCCTGGTTTCAACAGAAACCAGGGAAAGCCCCTAGATCGCTGATCTTTTCTGCTTC19330.22537753360258933No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC19170.22351201858052963No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18270.21301849658144373No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17980.20963725060396046No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17960.20940406122620303No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17610.2053232471154474No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17470.20369092147114512No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17400.20287475864899404No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17150.19995989142702572No Hit
AGTCAGGACATAGGTGATTATTTAGCCTGGTTTCAACAGAAACCAGGGAAAGCCCCTAGATCGCTGATCTTTT16930.19739480827169362No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16550.19296421009430179No Hit
CTCCAGACCCTTGCCCGGAGCCTGGCGGACCCAATGAAAAGAATAACTAC16220.1891165853613036No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15670.18270387747297334No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15100.17605798020688562No Hit
ATCTAGGGGCTTTCCCTGGTTTCTGTTGAAACCAGGCTAAATAATCACCT15050.17547500676249195No Hit
GAAATATAGAGCCGTGTCTTCGGGTCTCAGACTGTCCATCTGCAGATAGA14520.16929548825191915No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14460.16859592011864677No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14290.1666138104077083No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT14290.1666138104077083No Hit
CCTAAGAGGTGTCCATTCTCAGATTTTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGGGGTCCCTCAGACT14140.16486489007452731No Hit
GTCTGCATCTGTCGGTGACAGAGTCACCCTCACTTGTCGGGCGAGTCAGG13780.16066748127489294No Hit
GTCTGAGACCCGAAGACACGGCTCTATATTTCTGTGCGCGGGGGGGCTATTATGACAACGGTGGGAATCTCTACG13610.15868537156395454No Hit
CCCCTAGATCGCTGATCTTTTCTGCTTCCAGCTTGCAAAGTGGGGTCCCA13180.15367179994216904No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13120.15297223180889666No Hit
GACTTCACTCTCACCATCACCAGCCTCCAGCCCGAAGATTTTGCGACTTATTACTGCCAACAGTATCATACTTTC13030.15192287960898804No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13020.15180628492010934No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12690.14795866018711115No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGGCAGGTGTGACAT12670.1477254708093537No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12530.14609314516505145No Hit
GCACTGGAAGTCGCCGGTCTTTCCATTCGGTGATCAGCACTGAACGCAGA12370.14422763014299173No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12340.14387784607635554No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12290.14329487263196186No Hit
GTTTCCCAGGTGGCAGGTGTGACATCCAGATGACCCAGTCTCCATCGTCA12250.14282849387644694No Hit
CCTATGTCCTGACTCGCCCGACAAGTGAGGGTGACTCTGTCACCGACAGA12120.1413127629210234No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT11910.13886427445457006No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGGCAGGTGTGACATCCAGATGACCCAGTCTCCATCGTCA11870.1383978956990551No Hit
CACCATCACCAGCCTCCAGCCCGAAGATTTTGCGACTTATTACTGCCAACAGTATCATACTTTCCCCCTCACTTT11850.13816470632129765No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11490.1339672975216633No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11400.13291794532175472No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11300.1317519984329674No Hit
GAGTGAAGTCCGTCCCAGATCTACTGCCGCGGATCCTTGATGGGACCCCA11290.13163540374408866No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11260.13128561967745245No Hit
GGATGTGACTGTGCCCTGCCCAGTTACCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11200.13058605154418007No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11190.1304694568553013No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11090.129303509966514No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11050.12883713121099907No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11010.12837075245548416No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10990.12813756307772667No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC10980.12802096838884794No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC10910.12720480556669683No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10890.12697161618893935No Hit
CTATATTTCTGTGCGCGGGGGGGCTATTATGACAACGGTGGGAATCTCTA10780.12568907461127332No Hit
GTATCAACGCAGAGTACGGGAGGAATCAGACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTC10710.12487291178912219No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10680.12452312772248599No Hit
GTGCAGCCACAGTTCGTCTGACGTCCACCTTGGTCCCCCCGCCGAAAGTG10520.12265761270042626No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10510.12254101801154754No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10300.12009252954509415No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10250.11950955610070049No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10050.11717766232312585No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9880.1151955526121874No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9840.11472917385667249No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9840.11472917385667249No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9760.11379641634564264No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG9520.11099814381255306No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9280.10819987127946348No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGACGTCCACCTTGGTCC9240.10773349252394854No Hit
CTCCTAAGAGGTGTCCATTCTCAGATTTTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGGGGTCCCTCAGA9210.10738370845731235No Hit
GTCGGTGACAGAGTCACCCTCACTTGTCGGGCGAGTCAGGACATAGGTGATTATTTAGCCTGGTTTCAACAGAAA9200.10726711376843362No Hit
TCCTTGATGGGACCCCACTTTGCAAGCTGGAAGCAGAAAAGATCAGCGAT9180.10703392439067616No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGGGGTCCCTCAGACTCTCATGCT9120.10633435625740377No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9120.10633435625740377No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9080.10586797750188884No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9000.10493521999085899No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8970.10458543592422279No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8870.10341948903543545No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8850.103186299657678No Hit
GCACTGAACGCAGAGGGCGCACCATGGCGTTTGGGCTGGGCTGGCTTTTC8810.10271992090216306No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8750.10202035276889068No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8740.10190375808001194No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8670.1010875952578608No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8590.10015483774683095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA352.4198574E-548.764411
ACCGTAC1150.047.5338827
CGTACTA1200.045.59055729
TTGGCGT306.6653144E-445.4922183
CGTATGC1300.045.21574441
ATCTCGT1150.044.87211237
GTGGTAT1600.044.80231
GACCGTA1250.043.70820226
GGTATCA6400.042.1354941
GTACTAG1300.042.09588630
TGGTATC1800.039.8056872
TCGTATG1400.039.4766340
TACTAGA1400.039.12562631
CTTGTAT350.001423195638.993332
GTATGCC1450.038.18297642
ACGGGTC451.0689839E-437.9101816
GAGACCG1500.036.41075524
GTATCAA15500.036.3373451
GGAGCAT5550.036.12888770
TCCGAGC1550.035.2197813
Produced by FastQC (version 0.11.5)