FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857672
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATACTTTCCCCCTCACTTTCGGCGGGGGGACCAAGGTGGACGTCAGACGAACTGTGGCTGCACCAT48040.5601208853734295No Hit
GTATGATACTGTTGGCAGTAATAAGTCGCAAAATCTTCGGGCTGGAGGCTGGTGATGGTGAGAGTGAAGTCCGTC44790.5222276114878416No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31250.36435840274603815No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24940.2907871540635581No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20490.23890251751252228No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20230.2358710556016752No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC19130.22304563982501469No Hit
GGACATAGGTGATTATTTAGCCTGGTTTCAACAGAAACCAGGGAAAGCCCCTAGATCGCTGATCTTTTCTGCTTC19010.22164650355846993No Hit
GGTCTGGAGTGGGTGGCAGTCATCTCCTTTGATGGATCCAAGACGTTCTA18910.2204805566696826No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18300.21336828064807992No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18200.2122023337592926No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17030.19856075516048094No Hit
AGTCAGGACATAGGTGATTATTTAGCCTGGTTTCAACAGAAACCAGGGAAAGCCCCTAGATCGCTGATCTTTT16170.18853361191690995No Hit
CTCCAGACCCTTGCCCGGAGCCTGGCGGACCCAATGAAAAGAATAACTAC15970.1862017181393353No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15780.1839864190506394No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15570.18153793058418602No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15350.17897284742885392No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15170.17687414302903676No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15130.1764077642735218No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14840.17302651829603857No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14780.1723269501627662No Hit
GTCTGCATCTGTCGGTGACAGAGTCACCCTCACTTGTCGGGCGAGTCAGG13690.15961812907498438No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13670.15938493969722692No Hit
ATCTAGGGGCTTTCCCTGGTTTCTGTTGAAACCAGGCTAAATAATCACCT13630.15891856094171197No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13520.15763601936404592No Hit
GAAATATAGAGCCGTGTCTTCGGGTCTCAGACTGTCCATCTGCAGATAGA13460.15693645123077352No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13210.15402158400880522No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT13180.15367179994216904No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12860.1499407698980496No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12650.14749228143159623No Hit
GACTTCACTCTCACCATCACCAGCCTCCAGCCCGAAGATTTTGCGACTTATTACTGCCAACAGTATCATACTTTC12570.14655952392056637No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12550.1463263345428089No Hit
CCCCTAGATCGCTGATCTTTTCTGCTTCCAGCTTGCAAAGTGGGGTCCCA12410.14469400889850664No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12370.14422763014299173No Hit
GCACTGGAAGTCGCCGGTCTTTCCATTCGGTGATCAGCACTGAACGCAGA12320.14364465669859805No Hit
GTCTGAGACCCGAAGACACGGCTCTATATTTCTGTGCGCGGGGGGGCTATTATGACAACGGTGGGAATCTCTACG12300.1434114673208406No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12170.14189573636541708No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12160.14177914167653835No Hit
CACCATCACCAGCCTCCAGCCCGAAGATTTTGCGACTTATTACTGCCAACAGTATCATACTTTCCCCCTCACTTT12080.14084638416550851No Hit
GAGTGAAGTCCGTCCCAGATCTACTGCCGCGGATCCTTGATGGGACCCCA12030.14026341072111484No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11980.13968043727672116No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11910.13886427445457006No Hit
GTATCAACGCAGAGTACGGGAGGAATCAGACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTC11860.13828130101017638No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11710.1365323806769954No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT11690.13629919129923795No Hit
GTTTCCCAGGTGGCAGGTGTGACATCCAGATGACCCAGTCTCCATCGTCA11390.132801350632876No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGGCAGGTGTGACAT11290.13163540374408866No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGGCAGGTGTGACATCCAGATGACCCAGTCTCCATCGTCA11170.13023626747754388No Hit
CCTATGTCCTGACTCGCCCGACAAGTGAGGGTGACTCTGTCACCGACAGA11140.12988648341090767No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11100.12942010465539275No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC11030.12860394183324161No Hit
CCTAAGAGGTGTCCATTCTCAGATTTTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGGGGTCCCTCAGACT11030.12860394183324161No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10880.12685502150006064No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10720.1249895064780009No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10540.12289080207818373No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10340.12055890830060909No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC10310.1202091242339729No Hit
GTGCAGCCACAGTTCGTCTGACGTCCACCTTGGTCCCCCCGCCGAAAGTG10090.1176440410786408No Hit
CTATATTTCTGTGCGCGGGGGGGCTATTATGACAACGGTGGGAATCTCTA10060.11729425701200459No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9810.1143793897900363No Hit
GGATGTGACTGTGCCCTGCCCAGTTACCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9770.11391301103452135No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9740.11356322696788516No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9700.11309684821237023No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9680.11286365883461276No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9610.11204749601246163No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9520.11099814381255306No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9410.109715602234887No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9250.10785008721282728No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG9110.10621776156852503No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9090.10598457219076755No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9080.10586797750188884No Hit
TCCTTGATGGGACCCCACTTTGCAAGCTGGAAGCAGAAAAGATCAGCGAT9000.10493521999085899No Hit
GTGGGGTCCCATCAAGGATCCGCGGCAGTAGATCTGGGACGGACTTCACT8960.10446884123534404No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8860.10330289434655672No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8850.103186299657678No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8810.10271992090216306No Hit
GCACTGAACGCAGAGGGCGCACCATGGCGTTTGGGCTGGGCTGGCTTTTC8700.10143737932449702No Hit
CTCATGCTCCACCTCCAAATTCACCTTCAGTAGTTATTCTTTTCATTGGGTCCGCCAGGCTCCGGGCAAGGGT8690.10132078463561828No Hit
CTCCTAAGAGGTGTCCATTCTCAGATTTTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGGGGTCCCTCAGA8670.1010875952578608No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8610.10038802712458843No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8580.10003824305795223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA200.00775423351.0686578
GCTTGTA606.2664185E-945.426151
GTGGTAT1700.040.0818941
GTATCAA14000.039.6667631
GGTATCA6000.036.9087451
TGGTATC1850.036.810532
GGAGCAT6000.036.56344670
TGGACCG400.00277184734.04185
TATCAAC16300.033.423062
TCAACGC16600.032.809464
ATCAACG16650.032.7109343
CAACGCA16900.032.4303555
CGATTCA9450.030.95318270
CCGCGAA553.5194628E-430.9507059
AGAGTAC17900.030.62217711
TAACCGT450.00493157830.2629097
GCAGAGT18700.029.49429
CAGAGTA18700.029.494210
ATGACGC500.005834072429.23993766
AGTACTT5750.029.01630413
Produced by FastQC (version 0.11.5)