FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803741
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAGCCAGGGAGAGCCCCTAAACTCCTGATCTATAAAGCATC26980.33568027511350046No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25760.3205012560016224No Hit
GTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA25330.3151512738556326No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25330.3151512738556326No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17880.222459722721623No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16340.203299321547613No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15650.19471446647614093No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14820.18438775675248617No Hit
GTGTATTACTGTGCGAGAGATCGGGAGGTGGTGACTAAATACTACTTTGA14780.1838900839947197No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14370.17878893822761313No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13900.17294128332385683No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13870.17256802875553195No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13600.16920873764060812No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13410.16684479204121727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13370.16634711928345075No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13220.1644808464418264No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12480.15527390042314626No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC12160.1512925183610143No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11490.14295649966842552No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11270.1402192995007098No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCTGAGGGAAA11020.13710884476466922No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10810.13449606278639512No Hit
GTGGGAGACAGAGTCATCATTACTTGTCGGGCGAGTCAGGGTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAG10590.1317588626186794No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10490.13051468072426314No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT10250.1275286441776642No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTCACAGTTTC10220.1271553896093393No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10060.12516469857827336No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9960.12392051668385712No Hit
CTAATACCCTGACTCGCCCGACAAGTAATGATGACTCTGTCTCCCACAGA9810.12205424384223275No Hit
GTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAGCCAGGGAGAGCCCCT9790.1218054074633495No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9550.11881937091675054No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9510.11832169815898406No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9480.11794844359065917No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9370.11657984350680133No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT9350.11633100712791808No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9310.11583333437015157No Hit
CAGTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATA9230.1148379888546186No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8990.11185195230801963No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8880.11048335222416178No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATAGCATAAATTGGGTCCGCCAGGCTCCAGGGAAGGGG8820.10973684308751201No Hit
GAGTGAAATCTGTCCCAGAGCCACCGCCGCTGAACCTTGATGGGACCTCA8750.10886591576142064No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8710.10836824300365416No Hit
CCCCTAAACTCCTGATCTATAAAGCATCCATTTTGCAAAGTGAGGTCCCA8660.10774615205644605No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8630.10737289748812118No Hit
GGCTCACAGTTTCCCTCAGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT8470.10538220645705519No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC8450.10513337007817196No Hit
GTGAGGTCCCATCAAGGTTCAGCGGCGGTGGCTCTGGGACAGATTTCACT8370.10413802456263896No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8290.10314267904710597No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8250.10264500628933948No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8160.10152524258436486No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8120.10102756982659836No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8070.10040547887939025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACT200.00718727252.05851744
CTAGTAG200.00762123751.2923330
ACGCGTA1900.041.33709727
GACGCGT1900.041.3190826
CGTATGC2000.039.7630441
TCGTATG2000.039.7104540
TCTGGAG8750.038.32916370
AATCTCG2050.036.8601636
CGCGTAG2200.035.71133828
AGACGCG2200.035.67799825
CGAGACG2200.035.67133723
GTATCAA17750.035.3919141
CGTAGTA2250.034.95477330
ACGAGAC2250.034.88081422
CACGAGA2250.034.8786421
CCGTCTT2300.034.84019547
TTGAGCG400.002743086834.11404411
GAGACGC2350.033.398624
GTATGCC2400.033.16514242
ATGCCGT2450.032.5808444
Produced by FastQC (version 0.11.5)