FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803741
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24910.3099257098990844No Hit
GGGTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAGCCAGGGAGAGCCCCTAAACTCCTGATCTATAAAGCATC24720.30756176429969356No Hit
GTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA23730.29524436354497285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22650.28180719908527746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21170.2633933070479172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17240.2144969585973591No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15120.18812030243573488No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14860.1848854295102527No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14120.17567848349157253No Hit
GTGTATTACTGTGCGAGAGATCGGGAGGTGGTGACTAAATACTACTTTGA13880.17269244694497357No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13550.1685866466934No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13340.1659738647151259No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13330.16584944652568429No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13080.16273899178964368No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11620.14457393613116662No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11380.14158789958456766No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11340.14109022682680117No Hit
GTGGGAGACAGAGTCATCATTACTTGTCGGGCGAGTCAGGGTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAG11310.14071697225847632No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11280.14034371769015141No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11160.13885069941685196No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10980.1366111720069027No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10750.1337495536497454No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTCACAGTTTC10640.1323809535658875No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC10640.1323809535658875No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10330.1285239896931972No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10250.1275286441776642No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCTGAGGGAAA10250.1275286441776642No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10160.12640888047268958No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9720.12093448013725813No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT9670.12031238919005002No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9610.11956588005340028No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9410.11707751626456782No Hit
CTAATACCCTGACTCGCCCGACAAGTAATGATGACTCTGTCTCCCACAGA9240.1149624070440602No Hit
GAGTGAAATCTGTCCCAGAGCCACCGCCGCTGAACCTTGATGGGACCTCA9060.11272287963411098No Hit
GTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAGCCAGGGAGAGCCCCT8970.11160311592913637No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8890.11060777041360338No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8720.1084926611930958No Hit
CAGTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATA8480.10550662464649681No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8350.10388918818375571No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA8290.10314267904710597No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATAGCATAAATTGGGTCCGCCAGGCTCCAGGGAAGGGG8250.10264500628933948No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8200.10202291534213136No Hit
GTTTAGGGGCTCTCCCTGGCTTCTGCTGATACCAGGCTAGCCAGCTACTA8200.10202291534213136No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8100.10077873344771512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2100.040.4973531
TAAGGAC350.001449863438.8460163
GTATCAA18850.038.8001171
CCGTTTT400.002220981335.6144459
TATCAAC21800.032.7429122
ATCAACG22150.032.072083
TCAACGC22450.031.6434974
CAACGCA23050.030.8217225
TCTGGAG8200.030.81932670
TAGGTAC450.004916687530.28139122
AACGCAG23900.030.013746
AATGGTA3900.029.90751370
ATTCACT1600.029.741482
CGCAGAG25250.028.4072788
ATAGTAC605.9139926E-428.3252183
GACAGGT1353.601599E-1027.718191
AGAGTAC25950.027.64271411
GCAGAGT26250.027.4563089
CGGATGC500.00827074227.22269817
GTTGGAC500.00831607827.192213
Produced by FastQC (version 0.11.5)