FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605567
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28470.47013790381576276No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCACCTTCAGGGAGA27950.46155091013876254No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19790.3268011632073743No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16780.277095680577046No Hit
GTGTCTGGTGGCTCCATCAATAGTTTCTCATGGAGCTGGATCCGGCAGTC15980.2638849210739687No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13590.2244177770585253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13050.21550051439394816No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12900.21302349698712114No Hit
GTATATTACTGTGCGAGACGGGGACACGTAGACTTGAATTACTACTTTGA11960.19750085457100533No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCAATAGTTTCTCATGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGA11860.1958495096331207No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11540.19056520583188977No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11070.18280388462383187No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10570.17454715993440859No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10380.17140960455242774No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10040.1657950317636199No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAATTATTTACATTGGTATCAGCAGAAA9950.16430882131952368No Hit
GGGTATATCTCTAACAGTGGGACCACCAACTACAACCCCTCCCTCAGGAG9900.16348314885058135No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9650.1593547865058697No Hit
CTTTTGGCCAGGGGACCCAGCTGGAGATCAAACGAACTGTGGCTGCACCA9630.15902451751829277No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9190.15175859979160028No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9120.15060265833508102No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9090.1501072548537156No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT8950.1477953719406771No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8800.14531835453385009No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8720.14399727858354236No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8590.1418505301642923No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8500.1403643197201961No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8410.13887810927609992No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8400.13871297478231145No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8300.1370616298444268No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8180.1350800159189652No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8120.1340892089562344No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8020.13243786401834975No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7850.12963057762394584No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC7840.12946544313015734No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT7750.12797923268606115No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7740.1278140981922727No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7640.12616275325438803No Hit
CAGTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCACCTTCAGGGA7500.1238508703413495No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7390.12203439090967638No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7220.11922710451527246No Hit
ACTTACCACTGTCAACAGACTTACACAACTTTGTACACTTTTGGCCAGGGGACCCAGCTGGAGATCAAACGAA7090.1170803560960224No Hit
ACTTTGTACACTTTTGGCCAGGGGACCCAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC7010.11575928014571468No Hit
GTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACATATTTCATT6880.11361253172646461No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6760.11163091780100304No Hit
GGCCAAAAGTGTACAAAGTTGTGTAAGTCTGTTGACAGTGGTAAGTTGCA6710.1108052453320607No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTGGGTCCCCTGGCCAAAAGTG6600.10898876590038757No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6570.10849336241902219No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTGGGTCC6560.10832822792523371No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6530.1078328244438683No Hit
GGACTGGAGTGGATTGGGTATATCTCTAACAGTGGGACCACCAACTACAA6380.10535580703704132No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6370.10519067254325286No Hit
GTGGTAAGTTGCAGAATCTTCAGGTTGCAGACCGGTGATGGTGAGAATGAAATATGTCCCAGATCCACTGCCA6210.1025485206426374No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6090.1005669067171758No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6070.10023663772959887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGAT6800.054.45723370
AATGGTA1701.8189894E-1242.78782770
GTATCAA14900.041.9813731
GTAGACC350.001494178338.607223
TAGTACT350.001494178338.607224
TATCAAC17050.036.858432
GACAGGT1300.036.412951
ATCAACG17750.035.589333
AACGCAG18100.035.0877726
TCAACGC18100.034.901144
CAACGCA18400.034.5156945
GTCTAGG400.002864753633.8120271
GTGGTAT1300.033.8120231
AGAGTAC19050.033.5153211
ACGCAGA19300.032.9061557
CGCAGAG19300.032.9061558
GCAGAGT19800.032.24589
GAGTTTA2000.032.1214261
TCTAATG8250.031.96954768
ACAGGTA751.9514082E-631.557891
Produced by FastQC (version 0.11.5)