FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021526
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC44400.4346438563482476No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATATAACACTAATCACACTGCTGTCATAGGA34640.33910052215998415No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30430.2978876700152517No Hit
GTGTTATATTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC28920.2831058631889937No Hit
GACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATATAACACTAATCACA27880.27292501610335906No Hit
CTCCTGCACTGGGAGCAGCCACAACATCGGGGCAGGCTATGATGTACAGTGGTACCAGCAACTTCCAGGGACAGC26180.256283246828764No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25490.2495286463584872No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT24200.2369004802618827No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23760.23259319880257573No Hit
ATATAACACTAATCACACTGCTGTCATAGGACTGGCAGTAATAATCAGCC23370.22877538114546278No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23010.22525124176966616No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTC22560.22084606754992042No Hit
ATGATGTACAGTGGTACCAGCAACTTCCAGGGACAGCCCCCAAACTCCTCATCTATGATAATAGTTATCGACCCT22410.21937767614333847No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22360.21888821234114453No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22120.21653878609061344No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21090.20645583176541762No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20580.20146330098303908No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18730.18335314030186212No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18150.17767536019641203No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18140.17757746743597325No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17990.17610907602939133No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17980.17601118326895254No Hit
GATTATTACTGCCAGTCCTATGACAGCAGTGTGATTAGTGTTATATTCGG17940.17561961222719735No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17860.174836470143687No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT17710.17336807873710508No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17650.17278072217447232No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16730.16377458821410323No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16650.16299144613059285No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCTTCAGGCTGGAGCCCAG16050.15711788050426517No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15910.15574738185812206No Hit
ATGATAATAGTTATCGACCCTCAGGGGTCCCTGACCGATACTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGG15500.1517337786801315No Hit
GGCTATGATGTACAGTGGTACCAGCAACTTCCAGGGACAGCCCCCAAACTCCTCATCTATGATAATAGTTATCGA15470.15144010039881511No Hit
GGCCATCACTGGGCTCCAGCCTGAAGATGAGGCTGATTATTACTGCCAGT15030.14713281893950814No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14940.146251784095559No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14720.1440981433659055No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14440.14135714607361927No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14370.14067189675054773No Hit
ACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATATAACACTAATCACAC14210.139105612583527No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14030.1373435428956287No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG13880.13587515148904677No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13610.1332320469571993No Hit
GACCGATACTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGGCCATCACTGGGCTCCAGCCTGAAGATGAGG13480.131959441071495No Hit
CTATTATCATAGATGAGGAGTTTGGGGGCTGTCCCTGGAAGTTGCTGGTA13420.13137208450886223No Hit
GTGATGGCCAGGGAGGCTGAGGAGCCAGACTTGGAGCCAGAGTATCGGTC13390.13107840622754585No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13280.13000158586271912No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12770.12500905508034058No Hit
GTCCTATGACAGCAGTGTGATTAGTGTTATATTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGG12690.12422591299683022No Hit
GTGCAGGAGATGGTGACCGTCTGCCCTGGGGCCCCAGACACTGAGGGCGG12560.12295330711112591No Hit
GTAGTAAACGGCCGTGTCTGCGACGGTCACAGACTTCAGGGTCAGGGAGAACTGATTCTTGGACGTGTCTATGGA12300.12040809533971723No Hit
TCACTGGGCTCCAGCCTGAAGATGAGGCTGATTATTACTGCCAGTCCTAT12250.11991863153752327No Hit
GTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGGAGCAG12160.11903759669357411No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12060.11805866908918616No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12050.11796077632874738No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC11460.11218510346285851No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11340.11101039033759297No Hit
GTAATAATCAGCCTCATCTTCAGGCTGGAGCCCAGTGATGGCCAGGGAGG11330.11091249757715418No Hit
GCCCCCAAACTCCTCATCTATGATAATAGTTATCGACCCTCAGGGGTCCCTGACCGATACTCTGGCTCCAAGT11200.10963989169144985No Hit
GTTTGGGGGCTGTCCCTGGAAGTTGCTGGTACCACTGTACATCATAGCCT11190.10954199893101106No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11110.10875885684750071No Hit
GATGTACAGTGGTACCAGCAACTTCCAGGGACAGCCCCCAAACTCCTCAT11100.1086609640870619No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC11050.10817150028486794No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11040.10807360752442914No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10650.10425578986731615No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10560.103374755023367No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10380.1016126853354687No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATATAACACTAATCA10220.100046401168448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATA150.00207930271.2039151
AGGGGGG4450.053.89917870
TCGTATG3850.046.6084840
ATCTCGT3750.045.63567737
TCTCGTA3850.045.4279938
CGTATGC4000.044.99002541
ACGAGAC4050.043.84290322
TATACAC4300.043.6056563
GGTATCA10700.042.904251
CTTATAC4400.042.648021
TTATACA4450.042.1358072
GTATGCC4350.041.48346742
CGAGACG4300.041.29794323
TGGTATC3300.039.570722
ATCTCCG4500.039.43736610
CCGTCTT4550.039.429447
CCCACGA4250.039.39552319
GTGGTAT3500.039.2535861
ATGCCGT4550.039.11959544
CATCTCG4500.038.68966336
Produced by FastQC (version 0.11.5)