FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021526
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC41850.40968120243635503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41330.4045907788935377No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATATAACACTAATCACACTGCTGTCATAGGA30890.3023907369954362No Hit
GTGTTATATTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC29970.29338460303506714No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29460.28839207225268865No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTG26460.25902424412105024No Hit
CTCCTGCACTGGGAGCAGCCACAACATCGGGGCAGGCTATGATGTACAGTGGTACCAGCAACTTCCAGGGACAGC25120.24590661422225182No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24530.24013094135636295No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23460.22965641598941192No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22740.22260813723781872No Hit
GACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATATAACACTAATCACA21910.2144830381213988No Hit
ATGATGTACAGTGGTACCAGCAACTTCCAGGGACAGCCCCCAAACTCCTCATCTATGATAATAGTTATCGACCCT20880.20440008379620295No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20610.20175697926435548No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20520.20087594442040635No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20210.1978412688468037No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19490.1907929900952105No Hit
ATATAACACTAATCACACTGCTGTCATAGGACTGGCAGTAATAATCAGCC19440.19030352629301653No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTC19070.18668149415678112No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18570.18178685613484138No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17390.17023551040306364No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT17290.1692565827986757No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17100.16739662035033862No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16800.16445983753717477No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16770.1641661592558584No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16740.16387248097454202No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16600.16250198232839888No Hit
GATTATTACTGCCAGTCCTATGACAGCAGTGTGATTAGTGTTATATTCGG16430.1608378054009394No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAC16420.16073991264050058No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16120.15780312982733674No Hit
GGCTATGATGTACAGTGGTACCAGCAACTTCCAGGGACAGCCCCCAAACTCCTCATCTATGATAATAGTTATCGA15790.15457266873285652No Hit
GGCCATCACTGGGCTCCAGCCTGAAGATGAGGCTGATTATTACTGCCAGT15500.1517337786801315No Hit
ATGATAATAGTTATCGACCCTCAGGGGTCCCTGACCGATACTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGG15230.14909067414828403No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15100.14781806826257973No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14740.1442939288867831No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14590.1428255374802012No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCTTCAGGCTGGAGCCCAG14460.14155293159449686No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14420.14116136055274167No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG14400.14096557503186408No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAC14180.13881193430221062No Hit
GTCCTATGACAGCAGTGTGATTAGTGTTATATTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGG13900.13607093700992437No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13840.1354835804472916No Hit
GACCGATACTCTGGCTCCAAGTCTGGCTCCTCAGCCTCCCTGGCCATCACTGGGCTCCAGCCTGAAGATGAGG13330.13049104966491307No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13170.12892476549789236No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT13110.1283374089352596No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13080.12804373065394323No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12970.12696691028911647No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12400.12138702294410521No Hit
GTGTCTGGGGCCCCAGGGCAGACGGTCACCATCTCCTGCACTGGGAGCAG12040.11786288356830858No Hit
GTGCAGGAGATGGTGACCGTCTGCCCTGGGGCCCCAGACACTGAGGGCGG11890.11639449216172668No Hit
TCACTGGGCTCCAGCCTGAAGATGAGGCTGATTATTACTGCCAGTCCTAT11560.11316403106724644No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11460.11218510346285851No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11390.11149985413978696No Hit
GTAGTAAACGGCCGTGTCTGCGACGGTCACAGACTTCAGGGTCAGGGAGAACTGATTCTTGGACGTGTCTATGGA11330.11091249757715418No Hit
GTGATGGCCAGGGAGGCTGAGGAGCCAGACTTGGAGCCAGAGTATCGGTC11330.11091249757715418No Hit
ACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATATAACACTAATCACAC11330.11091249757715418No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC11300.11061881929583779No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11170.10934621341013348No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11110.10875885684750071No Hit
GCCCCCAAACTCCTCATCTATGATAATAGTTATCGACCCTCAGGGGTCCCTGACCGATACTCTGGCTCCAAGT10980.10748625096179637No Hit
GATGTACAGTGGTACCAGCAACTTCCAGGGACAGCCCCCAAACTCCTCAT10860.10631153783653084No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10700.10474525366951012No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC10250.10034007944976436No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10250.10034007944976436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)