FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765691
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24810.324021047654994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24760.3233680427221947No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17590.22972713535878048No Hit
GGCTAACAGTTTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT16810.219540258407112No Hit
GTTATAACCCCTCCCTCAAGAGTCGAGTCACCATATCGATAGACACGTCC15900.20765556863016543No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15820.2066107607376866No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15560.20321513508713046No Hit
GTCTCTGGTGACTCCGTCAGCAGTGAAACTTACTACTGGACCTGGATCCG15130.19759929266505682No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14900.19459546997418017No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14790.1931588591220218No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14660.19146104629674374No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTCCCA14380.18780421867306785No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14180.1851921989418708No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAATCCGTC13880.18127416934507523No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13850.18088236638539568No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13730.17931515454667743No Hit
GTTGGAGACAGAGTCACTATCACTTGTCGGGCGAGTCAGGATATTCACACCTGGTTAGCCTGGTATCAGCAGAAA13480.17605012988268115No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13400.1750053219902023No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13010.16991188351436806No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12170.15894140064334045No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11360.14836272073199241No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTCCCACATGCGGCGC11050.14431409014863697No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11010.14379168620239757No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10890.14222447436367935No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGGTCGGTC10800.14104906548464066No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCCTCAGGGAGA10720.14000425759216187No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10540.1376534398340845No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCCTCAGGGA10530.13752283884752467No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC10490.13700043490128525No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGAATGGGAAA10360.13530262207600716No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTG10220.13347420826416923No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10160.13269060234481012No Hit
GCCCCTAACCTCTTAATCTATGGTGCATCCATTTTGCAAACTGGGGTCCC10140.1324294003716904No Hit
GATTTCACTCTCACCATCAGTAGCCTGCAGGCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC10100.131906996425451No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9930.12968677965393352No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9800.12798896682865543No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9360.12224252342002193No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9310.12158951848722264No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9210.12028350862162414No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9060.11832449382322634No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8970.11714908494418767No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT8920.1164960800113884No Hit
GGTTAGGGGCAGTCCCTGGTTTCTGCTGATACCAGGCTAACCAGGTGTGA8890.11610427705170884No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTCCCACATGCGGCGCCC8870.11584307507858914No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8680.11336165633395194No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8390.10957422772371622No Hit
TCAGTAGCCTGCAGGCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCATTCACTTTCGGCC8100.10578679911348049No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8100.10578679911348049No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8080.10552559714036079No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8040.1050031931941214No Hit
GGATATGGGACGGATTTCACTCTCACCATCAGTAGCCTGCAGGCTGAAGA7970.10408898628820243No Hit
GGATATTCACACCTGGTTAGCCTGGTATCAGCAGAAACCAGGGACTGCCCCTAACCTCTTAATCTATGGTGCATC7820.10212997148980464No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7750.10121576458388566No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7740.1010851635973258No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7690.10043215866452654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG150.0024739568.1496115
AGCTTAA200.006599771353.1929657
GTATCAA16850.044.7178651
CGTATGC1950.042.49884441
CGAGACT2600.040.6621723
TATACAC2950.040.4303783
CTACGCT2600.039.40715428
TTATACA2800.038.945182
TCGTATG1950.038.9083540
GCCGTCT1700.038.8627446
TACGCTG2650.038.6914829
CTTATAC2750.038.434191
ACGAGAC2800.037.7503322
TATCAAC19700.037.7096062
ACACATC3000.037.4871836
ATGCCGT2050.037.2120644
CCCACGA2750.037.17737219
ATCAACG20000.036.973583
CAACGCA20050.036.8837855
GTATGCC2350.036.78793342
Produced by FastQC (version 0.11.5)